Circulating Free DNA
Conditions
Brief summary
Methylation detection of circulating free DNA to identify markers for diagnosis and differential diagnosis of ovarian endometriosis.
Detailed description
Methylation detection of circulating free DNA and FFPF samples from patients with ovarian endometriosis, ovarian cancer and other benign ovarian cysts will be performed to select markers for ovarian endometriosis diagnosis. The markers will be validated in patients with ovarian cysts found by ultrasound. The diagnosis accuracy of the markers will be evaluated by comparing with the pathological diagnosis.
Interventions
DIAGNOSTIC_TESTDNA extraction and methylation detection
DNA extraction and methylation detection
Sponsors
Peking Union Medical College Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
1\. Marker selection group: 1. patients with ovarian endometriosis: Inclusion Criteria: 1. pathologically diagnosed as ovarian endometriosis; 2. surgery was performed in PUMCH
Exclusion criteria
a. combined with other gynecological benign and malignant tumors; b. clinically suspected malignant tumors; c. combined with other systemic malignancies or malignancies history; d. pregnant patients 2. patients with ovarian cancer: Inclusion Criteria: 1. pathologically diagnosed as epithelial ovarian cancer; 2. surgery was performed in PUMCH
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The efficacy of cfDNA methylation in the diagnosis of ovarian endometriosis | 12 months | The sensitivity, specificity and accuracy are calculated according to standard definitions to evaluate the efficacy of cfDNA methylation in the diagnosis of ovarian endometriosis |
Countries
China
Contacts
Primary ContactShu Wang
Outcome results
None listed