Exercise, Nutrition Therapy, Resistance Training, Muscle Strength, Hypertrophy
Conditions
Keywords
Protein supplementation, Amino acids, Resistance exercise, Muscle strength, Muscle hypertrophy
Brief summary
The present project aims to investigate the effects of high-quality protein (whey protein) vs. low-quality protein (collagen) on muscle mass and function (e.g., strength and power) during a 10-wk resistance training program in young adults. It will be tested the hypothesis that leucine-matched collagen should promote similar gains on muscle function and mass compared to whey protein supplementation.
Detailed description
The purpose of this study will be to investigate the effects of whey protein vs. leucine-matched collagen supplementation on muscle mass and function after a 10-wk resistance training program. Initially, all participants will be submitted for a 2-wk familiarization period, and then randomized into two groups (N = 11 / group): whey protein group and leucine-matched collagen group. The following variables will be assessed from pre-to post-training: anthropometric, muscle thickness from vastus lateralis and biceps brachii muscles using ultrasound, training load, mean power and peak torque for unilateral arm flexion exercise using a isokinetic dynamometer, countermovement vertical jump, nutritional intake, and testosterone-to-cortisol ratio . Shapiro-Wilk and Levine tests will be used to test the normality and homogeneity of the measurements, respectively. Two-way ANOVA (group x time) tests for repeated measures will be performed to assess changes over time and between groups for all dependent variables. Violation of sphericity will adjust using Greenhouse- Geyser correction. The Bonferroni post-hoc test will detect specific differences between groups. When appropriate, the percentage of change (∆%) will be reported according to the following equation: ∆% = \[(pre-average post average) / pre-average mean\] × 100. Values will be express as mean ± standard deviation. O significance level α will be 0.05.
Interventions
DIETARY_SUPPLEMENTWhey protein supplementation
35 g of whey protein (7 d/wk) during a 10-wk resistance training program (3 d/wk; 3 sets of 8-12 repetitions with an interval of 1-2 minutes between sets and exercises) involving the leg press, leg extension, and arm flexion exercises. The load will be adjusted weekly.
DIETARY_SUPPLEMENTCollagen supplementation
35 g of collagen (7 d/wk) + 2 g of leucine during a 10-wk resistance training program (3 d/wk; 3 sets of 8-12 repetitions with an interval of 1-2 minutes between sets and exercises) involving the leg press, leg extension, and arm flexion exercises. The load will be adjusted weekly.
Sponsors
Universidade Estadual de Londrina
Universidade Norte do Paraná
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Men and women aged 18-35 years * To be eutrophic (not overweight or obese)
Exclusion criteria
* To present a history of musculoskeletal disorders * Be attending a systematized weight training program for at least six months * To make use of medicines that could affect muscle function. * To have used ergogenic supplements and anabolic steroids for at least six months before study * To have restrictions for exercises practice considering Physical Activity Readiness Questionnaire (PAR-Q) responses.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in vastus lateralis thickness | Baseline and after 10 weeks | Measured by ultrasonography pre- and post-training |
| Change in mean power | Baseline and after 10 weeks | Mean power using a isokinetic dynamometer (BIODEX) |
| Change in peak torque | Baseline and after 10 weeks | Peak torque using a isokinetic dynamometer (BIODEX) |
| Change in biceps brachii thickness | Baseline and after 10 weeks | Measured by ultrasonography pre- and post-training |
| Change in muscle strength by training load | Baseline, 5 and 10 weeks | Training load for leg press, knee extension, and arm flexion exercises |
| Change in vertical jump | Baseline and after 10 weeks | Countermovement vertical jump performance will be assessed using the Smart Jump instrument |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Dietary intake | Baseline, 2, 5, 10 weeks | Dietary intake to be assessed using 3-day diet recalls. |
| Change in testosterone-to-cortisol ratio | Baseline and after 10 weeks | Blood samples will be collected for analyse of the testosterone-to-cortisol ratio |
Countries
Brazil
Outcome results
None listed