Infectious Keratitis
Conditions
Keywords
Infectious Keratitis, surfactant protein D, single nucleotide polymorphisms, Met11Thr, rs721917
Brief summary
This study explores the association between single nucleotide polymorphism (Met11Thr) of surfactant protein D(SP-D) and susceptibility and prognosis of infectious keratitis.Met11Thr of SP-D of patients with keratitis and normal controls were compared.Allele and genotype frequencies of patients with keratitis and normal controls were determined by polymerase chain reaction with sequence specific primers(PCR-SSPs) .SP-D gene polymorphism of patients with keratitis and normal controls was detected by Sanger sequencing
Detailed description
Surfactant protein D(SP-D) plays an important role in the innate immune system of the eye in the lacrimal duct, cornea and conjunctiva.The single lipopolysaccharide on the surface of bacteria and the receptors on the surface of immune cells are linked by SP-D ,which acts as a bridge to neutralize viruses in the innate immune defense system and assists other immune cells in clearing bacteria and fungi.SP-D can also participate in the acquired immune response after combining with lipopolysaccharide on the surface of microorganisms.Some single nucleotide polymorphisms of SP - D can not only affect the plasma level of SP - D, which leads to the differences of pathogen defense capability, but also can affect the space configuration and structure of carbohydrate recognition domain of SP-D, causing the change of ability of SP - D combined with pathogens to influence its defensive role.The concentration of SP - D in ocular surface is closely related to the occurrence and progression of infectious keratitis.Association between single nucleotide polymorphisms of SP-D and the occurrence and development of infectious keratitis has yet to be confirmed by research.
Interventions
DRUGTreatment of bacterial keratitis
0.3% Levofloxacin Eye Drops or 0.3% Tobramycin Eye Drops QID
DRUGTreatment of fungal keratitis
1% Fluconazole Eye Drops or 0.25% Amphotericin B Eye Drops QID
DRUGTreatment of viral keratitis
0.1% Acyclovir eye drops QID ，Ganciclovir gel QN，Famiclovir tablets 0.25g TID
DIAGNOSTIC_TESTcorneal scraping
culture and sensitivity of corneal ulcerated infiltrating edge tissue
OTHERSequencing
polymerase chain reaction with sequence specific primers(PCR-SSP) and Sanger sequencing
Sponsors
Second Affiliated Hospital, School of Medicine, Zhejiang University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Clinical diagnosis of Infectious Keratitis. * Laboratory diagnosis of Infectious Keratitis.
Exclusion criteria
* Patients with topical or systemic long-term use of hormones, interferon, or immunosuppressive therapy. * Patients with basic diseases such as diabetes, autoimmune disease, sjogren's syndrome, blepharitis, eyelid defect or incomplete eyelid closure. * Patients with history of corneal myopia laser, cataract surgery and vitrectomy, or history of serious ocular trauma and drug allergies.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Base type | before the treatment | Base type of SNP（rs721917）:(Met and Thr) |
| Genotype | before the treatment | Genotype of SNP（rs721917）:( Met /Met , Thr/Thr and Met/Thr) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Grading of Keratitis | before the treatment | Grading of Keratitis： 1. cornea:transparency ,edema,turbidity,perforation 2. conjunctiva: hyperaemia 3. blephar:inflammation |
Countries
China
Outcome results
None listed