Dental Erosion
Conditions
Brief summary
The aim of this study is to investigate whether smoking is associated with changes in salivary composition and/or predisposition to erosion. Healthy volunteers are observationally wearing an intraoral device with both bovine tooth specimens (enamel and dentin) and resin specimens twice for two hours each. Afterwards, specimens are eroded extraorally and calcium release into the acid is measured. Total protein concentration and protein composition of the salivary pellicles on the resin samples are measured. Additionally, salivary parameters (unstimulated and stimulated saliva flow rate, pH, buffer capacity, total protein content and protein composition as well as concentration of inorganic calcium, phosphate, and fluoride) are measured.
Interventions
Use of fluoridated toothpaste
Wearing of an intraoral device with bovine tooth samples
OTHERWearing of an intraoral device with resin samples
Wearing of an intraoral device with resin samples
Sponsors
University of Göttingen
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
20 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Volunteers aged between 20 and 50 years who are able to give written consent
Exclusion criteria
* Non-fulfillment of the inclusion criteria * Intake of medications or diseases altering salivary secretion * Refusal to use fluoridated toothpastes * Known allergies to substances used in the study * Orthodontic treatment or malfunction which does not allow wearing an intraoral device
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Calcium release from bovine enamel and dentin specimens by extraoral erosion (nmol/Square Millimeter). | Immediately after the intraoral device has been worn once for two hours. |
Secondary
| Measure | Time frame |
|---|---|
| Determination of unstimulated and stimulated saliva flow rate (mL/min). | Saliva samples are collected for 5 min each on three visits. |
| Determination of salivary buffer capacity (pH). | Saliva samples are collected for 5 min each on three visits. |
| Determination of total protein content (mg/L) and protein composition (qualitatively) in saliva. | Saliva samples are collected for 5 min each on three visits. |
| Determination of inorganic calcium (mmol/L), phosphate (mmol/L) and fluoride (µmol/L) in saliva. | Saliva samples are collected for 5 min each on three visits. |
| Determination of total protein concentration (ng/Square Millimeter) and protein composition (qualitatively) within the salivary pellicles. | Immediately after the intraoral device with resin samples has been worn once for two hours. |
| Determination of saliva pH (pH). | Saliva samples are collected for 5 min each on three visits. |
Countries
Germany
Contacts
PRINCIPAL_INVESTIGATORAnnette Wiegand, Prof. Dr. med. dent.
Dept. of Prev. Dentistry, Periodontology and Cariology, University Medical Center Göttingen, Germany
Outcome results
None listed