Allergic Conjunctivitis, Dry Eye Syndrome, Infectious Keratoconjunctivitis, Mucous Membrane Pemphigoid
Conditions
Keywords
Lacrimal fingerprint, Metabolomics, Proteomics, Ocular surface
Brief summary
This study aims to obtain the lacrimal fingerprint for frequent pathologies of the ocular surface and establish a normative base for each of them.
Detailed description
This is an exploratory study. The investigators aim to: * Test and perfect a tear sample analysis technique by mass spectrometry with samples collected by Schirmer strips; * Obtain the lacrimal fingerprint for various pathologies of the ocular surface, notably different forms of dry eye syndrome, infectious keratitis/conjunctivitis, mucous membrane pemphigoid and allergic conjunctivitis, and establish a normative base for each of them. During regular clinics at the cornea service of the ophthalmology department of the CHUM (Centre Hospitalier de l'Université de Montréal), the investigators aim to recruit a few hundred patients with various pathologies of the ocular surface, notably different forms of dry eye syndrome, infectious keratitis/conjunctivitis, mucous membrane pemphigoid and allergic conjunctivitis, and collect a sample of their tears via Schirmer strip. A case report form will be completed for each patient, noting known ocular diagnoses and active topical ophthalmic medication. Schirmer strips will be sent in sterile tubes to UQAM (Université du Québec à Montréal)'s department of chemistry for analysis by mass spectrometry.
Interventions
DIAGNOSTIC_TESTTear sample collection via Schirmer strip and subsequent analysis by mass spectrometry
During regular consultations at the ophthalmology department of the CHUM, eligible patients will undergo a standard 5 minute Schirmer tear test. The Schirmer strips will serve as tear samples and will be sent to UQAM's Department of chemistry for mass spectrometry analysis.
Sponsors
Centre hospitalier de l'Université de Montréal (CHUM)
Université du Québec a Montréal
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Patients with healthy corneas or suffering from one of these pathologies: Dry eye syndrome; Infectious keratitis and/or conjunctivitis; Mucous membrane pemphigoid; Allergic conjunctivitis.
Exclusion criteria
* Patients younger than 18 years old; * Patients incapable of giving informed consent.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| To obtain the metabolomic and proteomic fingerprint of the studied ocular surface pathologies | 5 years | Raw data will be analysed with MarkerView software by Sciex to identify specific over- or under-expressed markers in these ocular pathologies. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| To objectify changes in metabolomic and proteomic profile associated with topical treatments taken by participants in this study | 5 years | Raw data will be analysed with the same software to identify specific changes in proteomic and/or metabolomic expression associated with the topical treatments taken by participants in this study. |
Countries
Canada
Contacts
CONTACTMarie-Claude Robert, MD, M.Sc
CONTACTMarie-Catherine Tessier
PRINCIPAL_INVESTIGATORMarie-Claude Robert, MD, M.Sc
Centre hospitalier de l'Université de Montréal (CHUM)
Outcome results
None listed