Infertility, Female
Conditions
Keywords
denudation timing ICSI timing oocyte aging
Brief summary
This study aiming to investigate if difference in the timing of cumulus removal post retrieval would have any critical impact on fertilization and embryonic development.
Detailed description
Patients under 37 year old who were treated with controlled ovarian stimulation for ICSI were randomly divided into 2 groups; Group A: Oocytes were denudated immediately after oocyte retrieval , Group B: Oocytes were denudated 2 hours after retrieval and both groups were microinjected at 4 hours from ovum pickup. Microinjection was performed on all matured oocytes only and they were cultured to blastocyst stage.The rates of maturation, fertilization, good quality blastulation, chemical pregnancy and clinical pregnancy were analysed and compared between the two groups.
Interventions
PROCEDUREdenudation time lag
The time between ovum pickup and denudation of oocyte
Sponsors
Hawaa Fertility Center
Ahmed Saad
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
24 Years to 37 Years
Healthy volunteers
No
Inclusion criteria
* Women 24-37 years old * With good ovarian reserve * Induction using Long agonist protocol * unexplained infertility
Exclusion criteria
* severe male factor * Testicular samples for ICSI * poor ovarian reserve * no of oocytes collected less than 4
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Day 5, good quality blastocyst | 120 hour post ovum pickup | Assessment were done according to gardener classification |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| clinical pregnancy | 6 weeks from the last normal menstrual period | Assessment was done by observation of a gestational sac at 6 wks |
Countries
Egypt
Outcome results
None listed