High-Grade Squamous Intraepithelial Lesions, Low Grade Squamous Intraepithelial Lesion, Oxidative Stress
Conditions
Keywords
thiol disulfide balance, preinvasive lesion, cervix, oxidative stress
Brief summary
The purpose of this study is to investigate Thiol-disulfide balance in patients with cervical preinvasive lesions
Detailed description
To date various oxidative stress paramaters were studied in patients with cervical preinvasive lesions, however dynamic thiol disulfide hemostasis has not been previously investigated. The thiol-disulfide balance in the circulation will be measured by the automated system created by Erel & Neselioglu(1). References 1- Erel O, Neselioglu S. A novel and automated assay for thiol/ disulphide homeostasis. Clin Biochem 2014;47:326-32. doi:10.1016/ j.clinbiochem.2014.09.026.
Interventions
DIAGNOSTIC_TESTDisulphide(μmol/L)
Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with sphectrophotometry
DIAGNOSTIC_TESTTotal Oxidant Capacity(μmolH2O2Equiv/L)
Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with sphectrophotometry
Sponsors
Muğla Sıtkı Koçman University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
• Histopathologically confirmed diagnosis of LGSIL or HGSIL
Exclusion criteria
* pulmonary disease * pulmonary hypertension * cardiac dysfunction * renal dysfunction * liver disease * chronic ishemia * systemic inflammation * other cervical biopy results than LGSIL or HGSIL * concomitant malignancy * patients who use vitamin A,C or E (antioxidant vitamins) * patients who use smoke • patients who use drink alcohol * patients who use addictive for any drug
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| native thiol/total thiol ratio | baseline (preoperative in the morning or preprandial for healthy volunteers) | native thiol/total thiol ratio X (100 ) |
| serum total thiol level | baseline (preoperative in the morning or preprandial for healthy volunteers) | serum total thiol level in μmol/L |
| the disulphide/native thiol ratio | baseline (preoperative in the morning or preprandial for healthy volunteers) | the disulphide/native thiol ratio X (100 ) |
| disulphide/total thiol ratio | baseline (preoperative in the morning or preprandial for healthy volunteers) | disulphide/total thiol ratio X (100 ) |
| serum native thiol level | baseline (preoperative in the morning or preprandial for healthy volunteers) | serum native thiol level in μmol/L |
| serum disulphide level | baseline (preoperative in the morning or preprandial for healthy volunteers) | serum disulphide level in μmol/L |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| total oxidant capacity (TOC) | baseline (preoperative in the morning or preprandial for healthy volunteers) | TOC in mmol H2O2 equivalent/L |
| Oxidative stress index (OSI) | baseline (preoperative in the morning or preprandial for healthy volunteers) | OSI in mmol trolox/L. |
| total antioxidant capacity (TAC) | baseline (preoperative in the morning or preprandial for healthy volunteers) | TAC in mmol trolox equivalent/L |
Countries
Turkey (Türkiye)
Outcome results
None listed