Stunting
Conditions
Keywords
amino acid intake, blood amino acid, stunting, linear growth, intestinal permeability, lactulose mannitol ratio
Brief summary
This study will be comprised of 2 phases. Phase 1 study is a comparative cross-sectional study which will be conducted among children aged 12-24 months living in agricultural area. A total of 172 healthy children consisted of 86 stunted children (Length for age z-score \<-2 Standard deviation) and 86 non-stunted children (Length for age z-score ≥0.5 Standard Deviation) will be recruited. Dietary intake, urine and dried blood spot from finger prick will be collected to assess amino acid intake, intestinal permeability and essential blood amino acid respectively. Qualitative study i.e. focus group discussion (FGD) and market survey will also be implemented. According to the result of phase 1 study, 2 types of food-based approaches will be developed, namely Complementary food recommendations (CFR) and Food multi-mix formula
Detailed description
Phase 1 study will recruit a total of 172 healthy children who are matched by sex and age. Aside of being stunted, children must have normal weight for length z-score (not wasted or overweight). Data collection will be performed as follows: 1. Dietary intake of the children will be collected by 9 days Estimated Food Record by visiting the respondents house 2. Urine will be collected once to measure intestinal permeability profile using Lactulose Mannitol ratio indicator. 3. Dried blood spot from finger prick will be collected to essential blood amino acid. To collect information on locally available and culturally accepted protein rich food sources, series of focus group discussion (FGD) and market survey will also be implemented. Food-based approaches will be developed according to the information attained from phase 1 study. Problem nutrients that are identified from dietary assessment will be fulfilled through optimization of locally available food by using linear programming approach. Problem nutrients that are not able to be fulfilled by CFR will be use as a basis to develop a Food multi-mix (FMM) formula. Data of intestinal permeability profile will be used to add other locally available food in FMM that are potential to improve intestinal permeability.
Interventions
None listed
Sponsors
Indonesia University
Nia Novita Wirawan
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
12 Months to 24 Months
Healthy volunteers
Yes
Inclusion criteria
1. Stunting group is defined as length for age Z-score (LAZ) \<-2.0 SD and non-stunting group as LAZ ≥-0.5 SD 2. Normal weight for length Z-score (WLZ ≥-2 SD to ≤+1 SD) 3. Group matching by age, gender 4. Singleton 5. Full term gestation 6. Normal birth weight 7. Living with mother 8. Not suffered from diarrhea at least in the last 1 month (as reported by the mothers) 9. Not suffering from fever during assessment (as measured by the health staff) 10. Not born from diabetic mothers (as reported by the mothers)
Exclusion criteria
1. Drink Ultra High Temperature (UHT) milk at least 15 days before urine collection 2. Taking any medication. 3. Had taken non-steroidal anti-inflammatory drugs within two weeks of the study, 4. Taken antibiotics within 4 weeks of the study, 5. Unable to drink the sugar solution 6. Refuse to participate until completion of the study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Amino acid intake | January - February 2020 | Amino acid intake will be assessed by estimated food record |
| Blood amino acid | January - April 2020 | Essential blood amino acid will be measured from finger prick dried blood spot and will be analyzed using LC-MS/MS |
| Intestinal Permeability | January-April 2020 | Intestinal permeability will be measured from urine and will be analyzed using HPLC |
Other
| Measure | Time frame | Description |
|---|---|---|
| Linear growth | December 2019 | Linear growth in terms of length will be measured using anthropometric measurements to define stunted and non-stunted group. |
Contacts
Primary ContactNia N Wirawan, MSc.
Backup ContactUmi Fahmida, Dr
Outcome results
None listed