Irisin, Polymorphism, Restriction Fragment Length, Uncoupling Protein-1
Conditions
Keywords
Beige fat cell, Fat tissue, Athletes, Sedentary people, Irisin
Brief summary
This study aims to determine the effect of an acute maximal exercise on serum uncoupling protein-1, irisin, interleukin 6 levels, and on blood lipid and lipoprotein concentrations. This study also evaluates the role of uncoupling protein-1-3826 A/G polymorphism on this eventual effect. Therefore, this study hypothesizes that: H01: Sedentary people and athletes' serum uncoupling protein 1, irisin, and interleukine 6 levels are different before and after an acute maximal exercise. H02: Sedentary people and athletes' basal serum uncoupling protein 1 levels are associated with uncoupling protein 1-3826 A/G polymorphism. H03: Uncoupling protein 1-3826 A/G polymorphism has a modifying role in the effect of maximal exercise on serum uncoupling protein 1 levels. H04: Sedentary people and athletes' basal serum lipid and lipoprotein levels are associated with uncoupling protein 1-3826 A/G polymorphism. H05: Uncoupling protein 1-3826 A / G polymorphism has a modifying role in the effect of maximal exercise on serum lipid and lipoprotein levels.
Interventions
OTHERYo-Yo intermittent recovery test (level 1)
The Yo-Yo intermittent recovery test (level 1) will be administered once.
Sponsors
Ege University
Eastern Mediterranean University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Athletes group: * Must be basketball, or volleyball, or handball players. * Must be subjects who have been training regularly for at least 3 months. * Sedentary group: \* Must be subjects who have not been training regularly for at least 3 months. * For both groups: * Healthy. * Non obese (body mass index \< 30). * Aged between 18 and 35 years.
Exclusion criteria
* For both groups: * Being regular consumer of cigarettes, alcohol, drugs and antioxidant substances. * Being obese (body mass index \> 30). * Having an acute or chronic injury which is limiting physical performance. * Having a disease (anemia, infection...) detected from the biochemical analysis of the blood samples. * Being injured or sick during the study.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change from the baseline serum uncoupling protein 1 level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Uncoupling protein 1 analyzes were performed in the laboratory. |
| Change from the baseline serum irisin level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Irisin analyzes were performed in the laboratory. |
| Uncoupling protein-1-3826 A/G polymorphism determination | 15 minutes before the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples were taken 15 minutes before the Yo-Yo intermittent recovery test (level 1). Polymorphism was determined in the laboratory. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change from the baseline serum interleukin-6 level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Interleukine-6 analyzes were performed in the laboratory. |
| Change from the baseline serum nitric oxide level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Nitric oxide analyzes were performed in the laboratory. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Change from the baseline at serum glucose level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Glucose analyzes were performed in the laboratory. |
| Change from the baseline serum urea level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Urea analyzes were performed in the laboratory. |
| Change from the baseline serum creatinine level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Creatinine analyzes were performed in the laboratory. |
| Change from the baseline serum total cholesterol level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Total cholesterol analyzes were performed in the laboratory. |
| Change from the baseline serum low density lipoprotein level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Low density lipoprotein analyzes were performed in the laboratory. |
| Change from the baseline serum C reactive protein level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. C reactive protein analyzes were performed in the laboratory. |
| Change from the baseline serum triglyceride level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Triglyceride analyzes were performed in the laboratory. |
| Change from the baseline serum total antioxidant level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Total antioxidant analyzes were performed in the laboratory. |
| Change from the baseline serum total oxidant level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Total oxidant analyzes were performed in the laboratory. |
| Change from the baseline heparin plasma thiobarbituric acid reactive substances level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Thiobarbituric acid reactive substances analyzes were performed in the laboratory. |
| Change from the baseline serum high density lipoprotein level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. High density lipoprotein analyzes were performed in the laboratory. |
| Change from the baseline serum creatine kinase level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Creatine kinase analyzes were performed in the laboratory. |
| Change from the baseline serum alanine aminotransferase level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Alanine aminotransferase analyzes were performed in the laboratory. |
| Change from the baseline serum aspartate aminotransferase level at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Aspartate aminotransferase analyzes were performed from serum samples in the Medical Biochemistry Laboratory of the Ege University Faculty of Medicine. |
| Change from the baseline hemogram test at 3 minutes post Yo-Yo intermittent recovery test (level 1) | 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1) | Volunteers' postprandial venous blood samples was taken 15 minutes before the Yo-Yo intermittent recovery test (level 1) and 3 minutes post test. Hemogram test analyzes were performed in the laboratory. |
Countries
Turkey (Türkiye)
Outcome results
None listed