Mitochondrial Biogenesis
Conditions
Keywords
Mitochondria, Exercise training, VO2, Aerobic exercise, Citrate Synthase, Blood flow restriction
Brief summary
This parallel-arm trial study investigates the effects of restricting arterial blood flow during cycling exercise on citrate synthase. Participants will be randomized to either a blood flow restricted or non-blood flow restricted exercise training group. Both groups will complete seven, 30-minute exercise sessions over a 14-day period.
Interventions
OTHERBFR Exercise Training
Participants will complete exercise training on a recumbent bike placed on a 45 degree incline (BFR).
OTHERCTL Exercise Training
Participants will complete exercise training on a recumbent bike placed on flat ground (CTL).
Sponsors
Brendon Gurd, PhD
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Investigator, Outcomes Assessor)
Masking description
Allocation concealment, assessor blinding
Eligibility
Sex/Gender
ALL
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* 3 hours of physical activity per week * No previous cycling training * No concurrent involvement in another training program * Body mass index \< 30 kg/m2
Exclusion criteria
* Presence of cardiovascular disease * Presence of metabolic disease * Taking regular oral medication * Current smoker
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Citrate Synthase | 3 days prior to start of exercise training and 3 days after completing exercise training | Fold change in citrate synthase maximal activity |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Nuclear respiratory factor, 1 (NRF1) | 3 days before training to 3 days after training | Fold change in NRF-1 protein |
| Maximal oxygen consumption (VO2peak) | 3 days before training to 3 days after training | Change in VO2peak (POST-PRE) |
| Heart rate (HR) | Every 5 minutes during each of the 7, 30-minute exercise sessions | Average HR |
| Revolutions per minute (RPM) | Every 5 minutes during each of the 7, 30-minute exercise sessions | Average RPM |
| Blood [lactate] | During all exercise sessions at 10 and 30 minutes | Average blood lactate concentration |
| Transcription factor A, mitochondrial (TFAM) | 3 days before training to 3 days after training | Fold change in TFAM protein |
| Nuclear Factor, Erythroid 2 Like 2 (NFE2L2) | 3 days before training to 3 days after training | Fold change in NFE2L2 |
| Cytochrome c oxidase (COX) | 3 days before training to 3 days after training | Fold change in COX |
| Electromyography (EMG) | Throughout each of the 7, 30-minute exercise sessions | Median frequency and average amplitude of EMG signal |
| Near-infrared spectroscopy (NIRS) | Throughout each of the 7, 30-minute exercise sessions | Deoxygenated hemoglobin and oxygenated hemoglobin |
| Rating of perceived exertion (RPE) | Every 5 minutes during each of the 7, 30-minute exercise sessions | Average RPE |
Countries
Canada
Outcome results
None listed