The Effect of ProHydrolase® on the Amino Acid, Intramuscular Anabolic Signaling, and Endocrine Response to Resistance Exercise in Trained Males

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT04144114

Enrollment

Registered

2019-10-30

Start date

2018-08-01

Completion date

2018-12-07

Last updated

2019-10-30

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Amino Acids

Keywords

Amino Acids, Intramuscular Anabolic Signaling, Endocrine Response, Resistance Training

Brief summary

To examine the amino acid absorption following acute resistance exercise between three supplemental treatments: 1) Whey Protein + ProHydrolase (WPH) 2) Whey Protein (W) and 3) Non-Caloric Placebo (PL). To examine three supplemental treatments (WPH, W, PL) in conjunction with acute resistance exercise on the mTORC1 complex pathway. To examine three supplemental treatments (WPH, W, PL) in conjunction with acute resistance exercise on circulating concentrations of endocrine biomarkers.

Interventions

DIETARY_SUPPLEMENTProHydrolase®

Subjects received 250mg of ProHydrolase® with 25g whey protein mixed together in a drink

DIETARY_SUPPLEMENTWhey protein

Subjects received 25g of whey protein powder mixed to together in a drink

DIETARY_SUPPLEMENTPlacebo

Placebo

Study design

Allocation

RANDOMIZED

Intervention model

CROSSOVER

Primary purpose

BASIC_SCIENCE

Masking

TRIPLE (Subject, Caregiver, Investigator)

Eligibility

Sex/Gender

ALL

Age

18 Years to 35 Years

Healthy volunteers

Yes

Inclusion criteria

* • At least one-year of resistance training experience * Free of any physical limitations (determined by health and activity questionnaire). * Between the ages of 18 and 35. * Ability to leg press a weight equivalent to 1.5 times their body mass

Exclusion criteria

* • Inability to perform physical exercise (determined by health and activity questionnaire) * Taking any other nutritional supplement or performance enhancing drug (determined from health and activity questionnaire). * Physical injury preventing the athlete from participating in offseason training * Any chronic illness that causes continuous medical care

Design outcomes

Primary

Measure	Time frame	Description
Amino acid absorption	4 weeks	To examine the amino acid absorption following acute resistance exercise between three supplemental treatments: 1) Whey Protein + ProHydrolase (WPH) 2) Whey Protein (W) and 3) Non-Caloric Placebo (PL)
mTORC1 pathway	4 weeks	To examine three supplemental treatments (WPH, W, PL) in conjunction with acute resistance exercise on the mTORC1 complex pathway
Endocrine markers	4 weeks	To examine three supplemental treatments (WPH, W, PL) in conjunction with acute resistance exercise on circulating concentrations of endocrine biomarkers.

Countries

United States

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026