Skeletal Muscle Physiology
Conditions
Brief summary
The purpose of the project is to investigate the proteome signature dynamics and remodelling in human skeletal muscle induced by exercise training
Interventions
OTHEREndurance training
Subjects perform a period of endurance training
OTHERResistance training
Subjects perform a period of resistance training
OTHERSprint training
Subjects perform a period of sprint training
Sponsors
Morten Hostrup, PhD
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men and women * VO2max \> 55 mL/min/kgLM for men and \> 50 for women (only applicable for endurance and sprint training interventions) * BMI \< 26
Exclusion criteria
* Chronic disease deemed by the medical doctor to pose a risk or to affect study outcomes * smoking * Use of prescription medicine deemed by the medical to affect study outcomes * Pregnancy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Anabolic signaling | Change in anabolic signaling from rest to 1 hour following resistance exercise both at baseline and following 8 weeks of resistance training | Western blotting of proteins related to anabolic signaling |
| Skeletal muscle proteome signature | Change from baseline at week 6 or 8 depending on the intervention | Pathway enrichment analysis of the muscle proteome |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Maximal oxygen consumption | Change from baseline at week 6 following endurance and sprint training | VO2max during incremental exercise |
| Body composition | Change from baseline at week 6 or 8 depending on the intervention | Lean and fat mass during dual X-ray absorptiometry |
| Satellite cells | Change from baseline at week 8 following resistance training | Immunohistochemistry to determine number of satellite cells |
| Fiber size | Change from baseline at week 8 following resistance training | Immunohistochemistry to determine fiber size |
| Myonuclei | Change from baseline at week 8 following resistance training | Immunohistochemistry to determine number of myonuclei |
| Skeletal muscle mitochondrial respiration | Change from baseline at week 6 following endurance and sprint training | High resolution respirometry |
Other
| Measure | Time frame | Description |
|---|---|---|
| Skeletal muscle mitochondrial supercomplexes | Change from baseline at week 6 following endurance and sprint training | Pathway enrichment analysis of mitochondrial supercomplexes |
Countries
Denmark
Outcome results
None listed