Quantification of NLRP3 (rs4612666) and CARD8 Gene (rs2043211) in Generalized Chronic Periodontitis Subjects

Quantification of NLRP3 (rs4612666) and CARD8 Gene (rs2043211) in Subgingival Plaque and Blood Samples of Generalized Chronic Periodontitis Subjects With and Without Coronary Heart Disease

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT04127487

Enrollment

Registered

2019-10-15

Start date

2019-10-01

Completion date

2020-12-01

Last updated

2019-12-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Coronary Artery Disease, Periodontal Diseases

Keywords

NLRP3 (rs4612666), CARD8 (rs20432111)

Brief summary

Quantification of NLRP3 (rs4612666) and CARD8 (rs20432111) will be analysed in the subgingival plaque and blood samples of generalized chronic periodontitis with and without coronary heart disease. The demographic and the periodontal parameters were assessed and correlated with the quantification of NLRP3 (rs4612666) and CARD8 (rs2043211) was analysed with RT-PCR

Detailed description

The study is done in order to assess the demographic variables such as age, height, weight and socio-economic status, BMI, personal habits, waist-hip ratio, lipid profile, use of medications, and family history of coronary artery disease in generalised chronic periodontitis subjects with and without coronary artery disease. To assess and compare the periodontal status of generalised chronic periodontitis subjects with and without coronary artery disease. To determine the expression of NLRP3 (rs4612666) and CARD8 gene (rs2043211) polymorphisms in subgingival tissue samples of generalised chronic gingivitis periodontitis subjects with and without coronary artery disease. To correlate the demographic variables and periodontal parameters and the expression of NLRP3 (rs4612666) and CARD8 (rs2043211) gene polymorphisms in generalised chronic periodontitis patients with coronary artery disease and compare them with systemically healthy chronic periodontitis patients

Interventions

None listed

Study design

Observational model

OTHER

Time perspective

OTHER

Eligibility

Sex/Gender

MALE

Age

30 Years to 65 Years

Inclusion criteria

* Patients willing to participate in the study * Patients within the age group of 30-65 years ( male ) * Patients should have ≥ 20 remaining natural teeth * No history of long term antibiotic use in the past 6 months * For group I generalized chronic periodontitis patients with coronary heart disease * For group II generalized chronic periodontitis patients without coronary heart disease

Exclusion criteria

* Patients with systemic conditions such as type I, and type II diabetes mellitus, respiratory diseases, renal disease, liver disease, rheumatoid arthritis, allergy, advanced malignancies and hiv infection will be excluded from the present investigation. * Patients on drugs such as corticosteroids, antibiotics within 3 months of investigation will be excluded. * Current smokers, individuals who quit smoking less than 6 months ago. * Patients who had undergone periodontal therapy within the previous 6 months

Design outcomes

Primary

Measure	Time frame	Description
Expression of NLRP3 (rs4612666) and CARD8 (rs20432111) gene	12 months	Expression of NLRP3 (rs4612666) and CARD8 (rs20432111) gene using RT-PCR

Secondary

Measure	Time frame	Description
Quantification of NLRP3 (rs4612666) and CARD8 (rs20432111) gene	12 months	Quantification of NLRP3 (rs4612666) and CARD8 (rs20432111) gene using RT-PCR

Countries

India

Contacts

Primary ContactJaideep Mahendra, MDS, PhD

jaideep_m_23@yahoo.co.in9444963973

Backup ContactAbirami Nayaki Rao, BDS

abirami.nayaki1791@gmail.com9176327362

Outcome results