Diagnostic Techniques and Procedures, Early Diagnosis
Conditions
Keywords
Circulating cancer cells, Biomedical polymer material, Cell affinity difference, White blood cell attachment
Brief summary
This culture system utilizes the special affinity difference of biomedical material coating for different cells to achieve the effect of isolating tumor cells from the blood sample. The coating of the system has the characteristic that to make the WBCs adhesion, but the cancer cells in the blood sample suspend in the culture medium, which achieves the effect of separating cancer cells from the blood. The supernatant with the cancer cells can further be isolated from the cultural system for related analysis and detection to achieve early diagnosis and screening.
Interventions
None listed
Sponsors
National Taiwan University Hospital
Study design
Observational model
OTHER
Time perspective
OTHER
Eligibility
Sex/Gender
ALL
Age
20 Years to 70 Years
Healthy volunteers
No
Inclusion criteria
* Patients with lung cancer are between the ages of 20 and 70 * Willing to sign the consent form under the informed consent
Exclusion criteria
* Vulnerable Subjects included minors, prisoners, aborigines, pregnant women, people with mental disorders, students, and subordinates, etc.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The Number of Circulating Cancer Cells from The Blood Sample | about 1 week | The number of circulating cancer cells from the blood sample will be investigated. To distinguish cancer cells from blood cells for the system, the lung cancer cells were immunofluorescence stained for Cytokeratin, white blood cells were stained for CD45 and nuclei were counterstained with DAPI. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Cancer Cell Isolation Efficiency of The Isolation System | about 1 week | The cell isolation efficiency was characterized by spiking A549 lung cancer cells with celltracker at the concentrations 1000, 100 and 10 cells into whole blood. |
Countries
Taiwan
Contacts
Primary ContactTai-Horng Young
Backup ContactYin-Tzu Chen
Outcome results
None listed