Blastocyst Disintegration
Conditions
Keywords
blastocyst, Vitrification, artificial collapse, survival rate
Brief summary
: This prospective randomized observational study aimed to compare behavior of artificially collapsed blastocysts to blastocysts that were vitrified without artificial collapse.
Detailed description
One arm study of 60 patients with sibling blastocysts divided into 2 groups: Study group (artificially laser-collapsed blastocysts; n=71) & control group (blastocysts were vitrified without laser collapse; n=66) then reexpansion speed and survival rate was assessed immediately & at 140 min after thawing and compared between two groups.
Interventions
DEVICElaser assisted collapse
Apply 1-2 pulses of laser using the Saturn between 2 adjacent trophectoderm cells away from the inner cell mass in the blastocysts
Sponsors
Hawaa Fertility Center
Benha University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Caregiver, Outcomes Assessor)
Masking description
only the investigator knew the procedure provided for each case
Intervention model description
patients with sibling blastocysts divided into 2 groups: Study group (artificially laser-collapsed blastocysts; n=71) & control group (blastocysts were vitrified without laser collapse; n=66) then reexpansion speed and survival rate was assessed immediately & at 140 min after thawing and compared between two groups.
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 37 Years
Healthy volunteers
No
Inclusion criteria
* Age limit 20-37 At least 6 good quality blastocysts per case. expansion rate 3-4
Exclusion criteria
* bad quality blastocysts expansion rate less than 3
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| survival rate | immediately after thawing | survival rate will be assessed immediately after thawing and compared between both groups. |
Countries
Egypt
Outcome results
None listed