The Role of Unsteady Wall Shears Stress Stimuli on Human Umbilical Vein Endothelial Cells Harvested From Umbilical Cords

The Role of Unsteady Wall Shears Stress Stimuli on Human Umbilical Vein Endothelial Cells (HUVECs) Harvested From Umbilical Cords

Status

Terminated

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT04122794

Acronym

HUVEC

Enrollment

Registered

2019-10-10

Start date

2019-11-20

Completion date

2024-03-22

Last updated

2024-04-12

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Vascular Diseases

Keywords

Endothelial Cells

Brief summary

The aim of this study is to obtain and test endothelial cells (EC's) from umbilical cords to examine specific properties of these cells which make up the inner lining of blood vessels.

Detailed description

Mechanical testing will be performed on the Ec's to see how the cells reacts and ultimately how the blood vessel is affected. A central role in the mechanism that defines the nature of vessel remodelling is played by endothelial cells. EC's are extremely sensitive to the frictional forces exerted on a unit of the endothelial area by blood flow. This mechanical stimulus is known as wall shear stress (WSS). WSS experienced by EC's in-vivo is unsteady due to the unsteady nature of blood flow produced during the cardiac cycle and due to the complex nature of the vascular tree. The extent to which this applies to venous cells and tissue remains unknown and it is the focus of this study.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

CROSS_SECTIONAL

Eligibility

Sex/Gender

FEMALE

Age

18 Years to 40 Years

Inclusion criteria

* Patients who are attending the Maternity Services who are pregnant and due for routine delivery. * Aged 18- 40 years. * Are willing and capable to voluntarily sign a statement of informed consent to take part in this investigation. * Patients due to give birth to twins will be included in this study. Women having both normal delivery and caesarean section delivery will be included in this study.

Exclusion criteria

* Patients unable to provide informed consent. * Patients who are under the age of 18 and over the age of 40 years. * Patients who are deemed unsuitable due to any circumstances as deemed appropriate by the Consultant. * Patients with a Blood Borne Virus (HIV Type 1, HIV Type 2, Hepatitis B, Hepatitis C) will be excluded from the study. * Patients with diabetes that is not gestational in nature will be excluded from this study. * Patients due to deliver triplets or more will be excluded from this study. * Patients who deliver their baby before 37 weeks gestation will not have umbilical cord tissue retained for study purposes.

Design outcomes

Primary

Measure	Time frame	Description
The identification of Endothelial Cell phenotype	2 years	To identify this phenotype by assessing the endothelial identity markers, the endothelial to mesenchymal transition markers and the regulation of genes involved in EC quiescence.

Countries

Ireland

Outcome results