Human Papilloma Virus Infection, Pterygium
Conditions
Keywords
HPV infection, Pterygium, Cytokine, VEGF, auto-inoculation
Brief summary
Prevalence of HPV-associated eye infection and cytokine levels in tears from patients diagnosed with pterygium
Detailed description
The main purpose of this study is to evaluate the prevalence of HPV infection in primary and recurrent pterygium and the association of HPV types collecting from conjunctiva swab and urine. The second purpose is to compare a quantity of cytokines including IL-6, IL-18 and growth factor including VEGF found in tears of patients with primary and recurrent pterygium to participants with free of ocular disease Primary hypothesis \- The prevalence of HPV infection in primary pterygium is difference with prevalence in recurrent pterygium patients. Secondary hypothesis * Ocular HPV infection has association with HPV genitalia infection. * Tears cytokine and growth factor level (IL-6, IL-18 and VEGF) are higher in pterygium HPV infection than non-HPV infection and also more than normal population
Interventions
DIAGNOSTIC_TESTHPV genotyping assay using HPV GenoArray Diagnostic Kits (Hybribio Ltd., Sheung Wan, Hong Kong)
HPV genotyping assay using HPV GenoArray Diagnostic Kits (Hybribio Ltd., Sheung Wan, Hong Kong) for swab sample collected from pterygium and normal conjunctiva and also for urine collected in Colli-PeeTM device (Novosanis, Belgium)
DIAGNOSTIC_TESTBio-Plex® 200 system (Bio- Rad, Hercules, CA)
Bioplex was use for measure cytokine IL-6, IL-18 and VEGF from tears collected from Schirmer strip
Sponsors
Chulalongkorn University
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Healthy volunteers
Yes
Inclusion criteria
Participants as primary and secondary pterygium group Inclusion Criteria: * Age above 18 years old * presence of pterygium invading the cornea
Exclusion criteria
* Other ocular inflammation that could affect the results of the study (such as conjunctivitis, keratitis, conjunctival tumor, etc) or would preclude the enrollment for safety reasons Participant without ocular disease Inclusion criteria * Age above 18 years old
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| HPV prevalence | 1 hour per participants which is on a day for collecting sample | 1\. To compare HPV prevalence in subjects with primary pterygium and with recurrent pterygium |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| HPV autoinoculation | 1 hour per participants which is on a day for collecting sample | To assess whether HPV autoinoculation is present, we evaluate whether there is an association between HPV ocular infection and genital infection. |
| cytokine IL-6, IL-18 and VEGF levels | 1 hour per participants which is on a day for collecting sample | To compare cytokine IL-6, IL-18 and VEGF levels among various subgroups, including recurrent pterygium subjects with HPV positive and HPV negative status, primary pterygium subjects with HPV positive and HPV negative, and subjects without ocular diseases |
Countries
Thailand
Outcome results
None listed