Serum Folic Acid Status, Genotyping MTHFR 677C-->T, Plasma Homocysteine, DNA Methylation of IGF2 Gene and Microbiota of the Children: Mother-Children Cohort Study

Serum Folic Acid Status, Genotyping MTHFR 677C-->T of the Mother and Serum Folic Acid Status, DNA Methylation of IGF2 Gene of the Children: East Jakarta Cohort Study

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT04096521

Enrollment

254

Registered

2019-09-20

Start date

2018-08-02

Completion date

2019-03-16

Last updated

2020-01-02

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Folic Acid Deficiency

Keywords

serum folic acid, genotyping, dna methylation of IGF2, East Jakarta Cohort Study, mother-children

Brief summary

This study is part of the East Jakarta Cohort Study to assess the current micronutrient status of the mothers and their children. The study also includes the assessment of genotyping of MTHFR 677C--\>T of the mother and DNA methylation of IGF2 gene of the children.

Detailed description

The initial study recruited 315 mothers in the public health centre and one district hospital in East Jakarta. The follow-up study is able to re-visit the 127 of them. This also includes 127 peers of the subject that lives in the same neighbourhood. The design of the study is case-cohort design, part study of the existing cohort. The assessment of the follow-up study includes the mother and the children born in the initial study. Anthropometric measurement, socioeconomic data from a structured questionnaire, biomarker assessment, blood pressure were obtained from the subjects.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

3 Years to 40 Years

Healthy volunteers

Inclusion criteria

* participant in the previous study * apparently healthy * data complete * voluntarily to participate

Exclusion criteria

* not complete data * migrate * refuse to participate in the study

Design outcomes

Primary

Measure	Time frame	Description
DNA methylation of IGF2 gene	December 2018 - March 2019	DNA methylation of IGF2 gene using pyrosequencing method
Serum folic acid status	December 2018 - March 2019	The measurement of serum folic acid status is using LC-MS/MS
Genotyping MTHFR 677C-->T	December 2018 - March 2019	The assessment of MTHFR 677C--\>T using Real Time PCR

Secondary

Measure	Time frame	Description
Blood pressure	December 2018-March 2019	The blood pressure measurement using semi automatic device (Omron HBP1300)
Serum Homocysteine	December 2018-March 2019	The measurement of serum homocysteine using Electrochemiluminescen
Serum Adiponectin	December 2018-March 2019	The measurement of serum adiponection using electrochemiluminescence

Other

Measure	Time frame	Description
Nutritional status assessment	September 2018-December 2018	Using antrhopometric measurement, using SECA for weight measurement, Shorrboard for height and standardized plastic tape for mid upper arm circumference.

Countries

Indonesia

Outcome results