Evaluation of Bacterial Flora Among Operation Theatre Staff at a Tertiary Care Hospital

Evaluation of Bacterial Flora Among Operation Theatre Staff at a Tertiary Care Hospital - An Observational Prospective Study

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT04089683

Enrollment

361

Registered

2019-09-13

Start date

2014-04-30

Completion date

2016-04-30

Last updated

2019-09-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Microbial Colonization

Keywords

Operation Theatre, Bacterial Contamination, Web-space, OT Dress, Anterior Nares

Brief summary

Health care associated infection (HCAI) is a serious health hazard as it leads to increased morbidity and mortality of patients, length of hospital stay and costs associated with increased hospital stay. A total of 361 subjects divided into four groups were included in this study. The first group comprised of 179 doctors, the second had 31 nurses, third group had 110 OT Technicians and the fourth group included 41 subjects which included housekeeping staff and cleaners. Swabs were collected from OT staff at the time of entry and at exit from the OT. Places of swabs taken were: A) Web space, B) OT dress and C) Anterior nares.

Detailed description

Introduction: Health care associated infection (HCAI) is a serious health hazard as it leads to increased morbidity and mortality of patients, length of hospital stay and costs associated with increased hospital stay. Infections from OT is well known and is well established which are often referred to as hot zones for emergence & spread of microbial resistance. Contamination in an operation theatre is an important cause of HCAIs and is the third leading cause of HCAIs. This was a prospective observational study conducted in the operation theatre of a tertiary hospital once a month randomly from April 2014 - April 2016. All Operation theatre (OT) staff were included in the study. All the patients in OT and samples from the OT air & equipment were excluded from the study. A total of 361 subjects divided into four groups were included in this study. The first group comprised of 179 doctors, the second had 31 nurses, third group had 110 OT Technicians and the fourth group included 41 subjects which included housekeeping staff and cleaners. Swabs were collected from OT staff at the time of entry and at exit from the OT. Places of swabs taken were: A) Web space, B) OT dress and C) Anterior nares.

Interventions

OTHERSwab stick

Swab sticks collected from Web Spaces, Scrub, Anterior Nares

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Healthy volunteers

Yes

Inclusion criteria

All Operation Theatre staff -

Exclusion criteria

Patients in Operation Theatre, Operating room air & equipment \-

Design outcomes

Primary

Measure	Time frame	Description
Bacterial flora harboured at Web spaces among OT staff	2 years	Swabs will be taken from web spaces before entering Operation Theatre and while going out of OT at the end of the day and cultured to look for growth of Bacteria
Bacterial load on OT dress among OT staff	2 years	Swabs will be taken from OT dress while donning them and before going out of OT at the end of the day and cultured to look for growth
To identify the nasal carriers of organisms among OT staff	2 years	Swabs will be taken from anterior nares while entering OT at the end of the day while leaving OT and cultured to look for growth

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026