Polymorphism, Restriction Fragment Length
Conditions
Keywords
VEGF gene expression, VEGF gene polymorphism, leptin
Brief summary
The aim of the study is to determine the relationship between VEGF gene expression, VEGF gene polymorphism and serum leptin concentration in the Polish population in people with excessive body weight. In addition, the aim of the study is to look for relationships between the VEGF gene polymorphism and anthropometric and biochemical factors of cardiovascular risk and endothelial dysfunction such as body weight, waist circumference, serum total cholesterol, LDL, HDL, triglycerides, glucose and the occurrence of cardiovascular diseases in the family of a patient with excessive body weight in the Polish population.
Detailed description
400 people (250 - study group; 150 control group) were subjected to subjective and objective action. Information on the occurrence of diseases and cardiovascular risk in the participant and his family was collected from research studies. Anthropometric parameters were measured (body weight, height, BMI, waist circumference, neck circumference) as well as blood pressure and pulse measurement. In addition, fasting venous blood was collected and secured. In venous blood currently marked with the following concentration: glucose, required cholesterol, LDL cholesterol, HDL cholesterol and triglycerides. In addition, a method of salting out and protecting DNA was developed from blood. Furthermore the polymorphisms of VEGF genes (in positions: -2578 and -634) using the HMR (High Resolution Melt) method was determined. Also VEGF and leptin by ELISA was determined. After obtaining the results of comparative analysis of the correlation between the occurrence of VEGF gene polymorphisms, serum levels of leptin and VEGF, and anthropometric and biochemical parameters of cardiovascular risk.
Interventions
GENETICgene expression
determination of VEGF gene expression, VEGF gene polymorphism and serum leptin concentration
Sponsors
Poznan University of Medical Sciences
Study design
Observational model
COHORT
Time perspective
OTHER
Eligibility
Sex/Gender
ALL
Healthy volunteers
Yes
Inclusion criteria
* informed consent in writing * stable body mass (+/- 1 kg) * excessive body mass (BMI ≥ 25 kg/m2- study group) * proper body mass (BMI \< 25 kg/m2- control group)
Exclusion criteria
* age \<18 years * secondary obesity * pregnancy, lactation * other conditions that in the opinion of researchers may pose any risk to the patient during the study.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| VEGF gene expression | through study completion, an average of 1 year | VEGF gene expression |
| VEGF gene polymorphism | through study completion, an average of 1 year | VEGF gene polymorphism |
| serum leptin concentration | through study completion, an average of 1 year | serum leptin concentration |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| low density lipoprotein (LDL) | through study completion, an average of 1 year | Blood concentration of low density lipoprotein |
| high density lipoprotein (HDL) | through study completion, an average of 1 year | Blood concentration of high density lipoprotein |
| Body mass (BM) | through study completion, an average of 1 year | Body mass |
| glucose | through study completion, an average of 1 year | Blood concentration of glucose |
| triglycerides | through study completion, an average of 1 year | Blood concentration of triglycerides |
| Waist circumference (WC) | through study completion, an average of 1 year | Waist circumference |
| total cholesterol (TCH) | through study completion, an average of 1 year | Blood concentration of total cholesterol |
Countries
Poland
Outcome results
None listed