Dehydration, Diabetes Mellitus, Type 2
Conditions
Keywords
dehydration, vasopressin, copeptin
Brief summary
Previous studies have indicated increased vasopressin due to hypertonic saline infusion impairs glucose regulation. The current study will examine the effect of low water intake on glucose regulation. No currently published study has investigated the acute effect of low water intake on glucose regulation using continuous glucose monitoring over the course of a full day. The aim of the study is to observe the effect of low water intake on glucose regulation in low drinkers. The study will study the glycemic responses to standardized meals in adults during an 11-hour period in two conditions: a) high water intake and b) low water intake. It is hypothesized that the area under the glucose curve will be greater in the low water intake trial as compared to the high water intake trial
Interventions
OTHERWater Intake
Increased water intake during the 11 h protocol
Sponsors
Arizona State University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
30 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy sedentary participants * aged 30-55 years * body mass 27.5-35.0 kg·m2 * low water intake less than 1.5 L∙day-1 (male) or 1.0 L∙day-1 (female) * 24 h urine osmolality greater than 800 mmol/kg
Exclusion criteria
* diabetes HbA1c \>6.5% * impaired liver or kidney function * cardiovascular disease * weight change of more than 3 kg in the past month * pregnancy * previous surgery on digestive tract * exercise \>4 h/week
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Area Under the Curve for Plasma osmolality | the entire 11-hour protocol 7:00 am - 6:00 pm |
| Area under the curve of Glucose at lunch | 2-hour window starting right before the lunch meal (12:00 pm - 2:00 pm) |
| Area under the curve of Glucose at Dinner | 2-hour window starting right before dinner (4:00 pm - 6:00 pm) |
| Area under the curve of Glucose for the entire experiment | 11-hour window starting right before breakfast till 2 hours after dinner (7:00 am - 6:00 pm) |
| Area under the curve of Copeptin for the entire experiment | 11-hour window starting right before breakfast till 2 hours after dinner (7:00 am - 6:00 pm) |
| Area under the curve of Glucagon at lunch | 2-hour window starting right before the lunch meal (12:00 pm - 2:00 pm) |
| Area under the curve of Glucagon at dinner | 2-hour window starting right before dinner (4:00 pm - 6:00 pm) |
| Urinary glucose excretion | Entire 11-hour protocol 7:00 am - 6:00 pm |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Area under the curve of Insulin at dinner | 2-hour window starting right before dinner (4:00 pm - 6:00 pm) | — |
| Area under the curve of Insulin at lunch | 2-hour window starting right before the lunch meal (12:00 pm - 2:00 pm) | — |
| Area under the curve of Glucagon like peptide 1at lunch | 2-hour window starting right before the lunch meal (12:00 pm - 2:00 pm) | — |
| Area under the curve of plasma cortisol | the entire 11-hour protocol 7:00 am - 6:00 pm | — |
| Area under the curve for Thirst rating | the entire 11-hour protocol 7:00 am - 6:00 pm | Thirst assessed by Visual analog scale |
| Mood assessment with a questionnaire | single measurement at baseline before the beginning of breakfast at 7:00 am and 1-hour before dinner meal at 3:00 pm | Assessed by Profile of mood state questionnaire |
| Area under the curve of Glucagon like peptide 1 at dinner | 2-hour window starting right before dinner (4:00 pm - 6:00 pm) | — |
Countries
United States
Outcome results
None listed