COPD Exacerbation, Smoking, Tobacco
Conditions
Brief summary
The purpose of the study is to explore the role of congenital mucosal immune system in the airways of patients with COPD including the impact of ILCs (Innate lymphoid cells) on metabolism of epithelial cells.During the research the following methods will be applied: measurement of Volatile Organic Compounds (VOCS), airway epithelial cytology and culture.
Detailed description
ILCs profiles, epithelium metabolism measured by VOCS and microbiome profiling (specific 16S rRNA-16S ribosomal RNA gene-targeted qPCR) will by determined in the airways of patients with COPD after the exacerbation. Microbiome profiling will be based on next generation sequencing (NGS) of the 16S rRNA encoding region.
Interventions
DIAGNOSTIC_TESTairway epithelial smear test
all patients will be subjected to all scheduled procedures: airways epithelial smear test, measurement of VOCs in exhaled air and airways slide sample culture
Sponsors
Medical University of Lodz
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Exclusion criteria
* Neoplasm * Diabetes * Astma * acute airway infection in last 2 weeks
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| percentage of nasal ILCs (Innate lymphoid cells) after COPD exacerbation | through study completion, an average of 1 year | The assessment of the nasal mucosa material after COPD exacerbation will include: percentage of ILC1, 2 and 3 cells (immunophenotyping and measurement by flow cytometry) |
Countries
Poland
Contacts
Primary ContactPaweł Majak
Outcome results
None listed