Interest of Rapid Typing in Adenovirus Infections.

Interest of Rapid Typing in Adenovirus Infections: Sensitivity and Specificity, Study of the Association Clinical Characteristics and Viral Type.

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT04056546

Acronym

TYPADENO

Enrollment

296

Registered

2019-08-14

Start date

2012-05-29

Completion date

2014-07-04

Last updated

2019-08-14

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Adenovirus, Adenovirus Disease, Genotyping

Keywords

Real-time Polymerase-Chain Reaction

Brief summary

Proposal of a rapid typing technique by a new real-time PCR method, simpler, faster and cheaper than nucleotide sequencing (reference method) for rapid typing in Adenovirus infections.

Detailed description

Adenoviruses cause many infections, mainly respiratory and gastroenteric, in pediatrics. They also behave as opportunistic agents in 10% of hematopoietic stem cell recipients. In these immunocompromised persons, these infections are all the more frequent as the graft is of placental origin and the recipient a child (20%). They are then willingly disseminated and potentially deadly; their treatment is based on cidofovir, a viral agent that is very sensitive to handling. Adenoviruses, which have a high genetic diversity, are classified into 7 types. According to some observations, still limited, some types of Adenovirus would show a higher pathogenicity (types C and A). Thus, viral typing may be prognostic for immunocompromised patients, justifying the initiation of an earlier specific treatment when identifying certain more pathogenic viral types, in addition to measuring the viral load, already performed by quantitative PCR. For this purpose, this study proposes a fast typing technique by new real-time PCR method, simpler, faster and cheaper than nucleotide sequencing, reference method.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

OTHER

Eligibility

Sex/Gender

ALL

Healthy volunteers

Inclusion criteria

\- viral load greater than or equal to 10 \^ 4 copies / mL (blood and body fluids) or copies / gram of stool or copies / μg of DNA (biopsies).

Exclusion criteria

* Samples identified as positive in Adenovirus, not re-analyzable (insufficient sample quantity, alteration during thawing).

Design outcomes

Primary

Measure	Time frame	Description
Type viral characterization (scale A to G)	At the screening	sensitivity and specificity of the rapid typing technique, expressed for each strain, relative to the reference method (nucleotide sequencing), among initial diagnostic samples of viral load\> 10 \^ 4 / mL.

Outcome results