Healthy
Conditions
Brief summary
This is a single-center, randomized, placebo-controlled pilot study to evaluate the effects of high intensity interval training (HIIT) and daily administration of BasisTM for 3 weeks, in 18-35 year old males. The study will consist of a screening visit, baseline visit, followed by treatment and HIIT phase (3 weeks) and a post-supplementation, follow-up visit (this will occur 48 h (or more) after the final HIIT session). Following the screening phase, subjects who meet entry criteria will be randomly assigned to 1 of 2 treatment groups: recommended dose of BasisTM (250 mg Nicotinamide Riboside and 50 mg Pterostilbene) per day or matching-placebo in a 1:1 ratio.
Interventions
DIETARY_SUPPLEMENTNRPT
Subjects randomly assigned to the active group will receive Nicotinamide Riboside and Pterostilbene (each capsule contains 125 mg NR and 25 mg PT, total daily dose is 2 capsules) for 3 weeks
DIETARY_SUPPLEMENTPlacebo oral capsule
Subjects randomly assigned to the Placebo group will receive placebo oral capsules for 3 weeks
Sponsors
University of Exeter
Elysium Health
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* 18 to 35 year old males * Body mass index between 18.5-30 kg.m-2 * Ability to give informed consent * Recreationally active (defined in the present study as exercising once a week or more).
Exclusion criteria
* Those regularly conducting HIIT or highly trained in endurance events (e.g. triathlete) * Currently taking supplements known to affect metabolism (e.g. ibuprofen, stimulants, vitamins, NR) * Taking prescribed medication * Consuming a vegan diet * Consuming a high fat, low carbohydrate diet * Habitual consumption of protein and/or dairy supplements (e.g. whey protein) * Smokers
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Mitochondrial function | 3 weeks | Measured via high-resolution respirometry using the Oroboros Oxygraph |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Muscle strength | 3 weeks | To measure maximal voluntary contraction using a force transducer strength chair |
| Muscle power | 3 weeks | Determine the effects of HIIT with and without NRPT supplementation on muscle power producing capabilities measured by the 30 second Wingate test |
| Mitochondrial proteins | 3 weeks | To determine whether HIIT with NRPT supplementation improves mitochondrial function more so than HIIT alone, using Western blotting |
| Body Mass | 3 weeks | To determine the effects of HIIT with and without NRPT supplementation on body composition by measuring body mass using a bioelectrical impedance analysis scale |
| Percent Body Fat | 3 weeks | To determine the effects of HIIT with and without NRPT supplementation on body composition by measuring percent body fat using a bioelectrical impedance analysis scale |
| Exercise Capacity | 3 weeks | To measure maximal endurance capacity measured via VO2max |
| Recovery via RESTQ | 3 weeks | To determine the effects of HIIT with and without NRPT supplementation on recovery via recovery stress (RESTQ) questionnaire |
| Physical activity levels using FitBit | 3 weeks | To characterize volunteer's habitual physical activity levels. |
| Height | 3 weeks | Measure height using a stadiometer, to characterize demographics of recruited volunteers the recruited volunteers |
| Blood pressure | 3 weeks | Measure blood pressure using Dinamap Pro monitor to characterize demographics of recruited volunteers the recruited volunteers |
| Recovery via POMS | 3 weeks | To determine the effects of HIIT with and without NRPT supplementation on recovery via profile of mood states (POMS) |
Countries
United Kingdom
Outcome results
None listed