COPD, Metabolomics, Omega 3, Omega 6
Conditions
Keywords
Metabolomics, Omega 6, Omega 3, COPD, lung function tests
Brief summary
Targeted metabolomics research was conducted on patients with chronic obstructive pulmonary disease (COPD). The correlation analyst between inflammation-related metabolites and lung function was analyzed by explored the metabolic pathways of inflammation-related omega-6 and omega-3 and combining them with lung function.
Detailed description
Based on the global initiative for chronic obstructive pulmonary disease, the 80 confirmed stable COPD patients with mild-to-moderate were involved, 80 COPD patients with severe and very severe, and age, sex, all matching 40 cases without lung disease. The participants information was collected. Whole blood and pulmonary function were examined at three time points: admission, six months and one year, at the same time, targeted metabolomic analysis method are applied to detect serum metabolites, According to the access to assess the different severity of COPD in the stable period and the level of metabolites in different admission periods. After that, correlation analysis was conducted with pulmonary function indicators and other clinical indicators to observe the research value of inflammation-related metabolites in the development of COPD.
Interventions
DIAGNOSTIC_TESTLung function test was performed on the subjects with Jaeger lung function instrument of Germany.
This Jaeger lung function instrument of Germany is used to test the pulmonary ventilation function of COPD patients. Since the condition requires drug control and treatment, the drug cannot be stopped.
DIAGNOSTIC_TESTAgilent 1290 Infinity LC system (UHPLC, Santa Clara CA) was used for Targeted metabolite detection.
We used the Agilent 1290 Infinity LC system mass spectrometer to analyze and detect metabolite peaks of patients according to retention time and mass/charge ratio after preliminary experiments and derivatization.
DIAGNOSTIC_TESTRoutine blood test
Blood routine test results include CRP, PCT, white blood cell count, red blood cell count, neutrophil percentage, eosinophil, basophil, et al.
Sponsors
The Fifth Affiliated Hospital of Zunyi Medical College
Guangzhou Institute of Respiratory Disease
Study design
Observational model
CASE_CONTROL
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
40 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* COPD diagnosis * The age ranges from 40 to 80 * No other pulmonary diseases * No immune-related diseases
Exclusion criteria
* Not consistent with COPD diagnosis * Age is out of range * Interstitial lung disease, asthma and other lung diseases * Immune system diseases * Lost to follow-up
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Forced vital capacity (FVC) | October 1, 2016 to October 1, 2018 | FVC is an important index to determine the resistance of respiratory tract. FEV1 and FEV1/FVC% in patients with obstructive airway obstruction, such as patients with chronic obstructive pulmonary disease, were reduced due to airway obstruction and prolonged expiratory. |
| The metabolites of Omega-6 inflammatory indicator | October 1, 2016 to October 1, 2018 | Agilent 1290 Infinity LC system (UHPLC, Santa Clara CA) was used for the targeted determination of metabolites in the serum of subjects. Omega6 metabolites are closely related to inflammation and have been shown to be involved in a variety of diseases such as COPD, rheumatism, cardiovascular disease, et al. |
| The metabolites of Omega-3 inflammatory indicator | October 1, 2016 to October 1, 2018 | As an important anti-inflammatory factor, omega-3 is widely used in chronic inflammatory diseases, which can fight against the pro-inflammatory metabolites of omega-6. |
| Forced expiratory volume in 1 second (FEV1) | October 1, 2016 to October 1, 2018 | The FEV1 percentage of predicted value is an important indicator in the diagnosis and treatment of COPD, and plays an important role in the severity rating. * level (mild) : FEV1 / FVC \< 70%, FEV1% pred \> 80%. Ⅱ level (moderate) : FEV1 / FVC \< 70%, 50% ≦ FEV1% pred\< 80%. Ⅲ level (severe) : FEV1 / FVC \< 70%, 30% ≦ FEV1% pred \< 50%. IV level (very severe) : FEV1/FVC\<70%, FEV1% pred \< 30%, or FEV1% pred \<50% with chronic respiratory failure. |
| FEV1/FVC ratio | October 1, 2016 to October 1, 2018 | This index is an important basis for the diagnosis of COPD and is crucial in the early stage of the disease to the later stage of the evaluation. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Leukocyte Count | October 1, 2016 to October 1, 2018 | White blood cells are very important blood cells in human blood. White blood cells bear many important tasks in the human body. They have the function of swallowing foreign bodies and producing antibodies. |
| C-reaction protein | October 1, 2016 to October 1, 2018 | CRP is an acute phase protein synthesized by liver cells in response to inflammatory stimuli such as microbial invasion or tissue injury. Can reflect the inflammation level in COPD patients. |
Outcome results
None listed