Urea in Blood; High
Conditions
Brief summary
Antibacterial properties of urea was tested against 35 isolates from ocular infections.
Detailed description
In vitro susceptibility testing was performed with commercially available antibiotic discs, homemade urea discs saturated with urea solution at a concentration of 96 mmol. The weight of urea in one drop = 0.37 mg, in one ml = 10.34 mg (5 microliter/ disc) discs and combined discs (antibiotic discs to which urea solution was added). Combined discs provided better in vitro susceptibility results than antibiotics alone. Scanning electron micrographs of bacterial isolates incubated overnight with urea solution showed marked disruption of bacterial aggregative patterns and irregularities of their cell walls. Western blotting showed marked reduction in total proteins in bacterial isolates incubated overnight with urea solution. These findings may explain the possible mechanisms for antibacterial activity of urea solution as a result of protein denaturation.
Interventions
BIOLOGICALAntibacterial Activity
Antibacterial Activity of Urea Solution against Ocular Bacterial Isolates
Sponsors
Assiut University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
5 Months to 65 Years
Healthy volunteers
No
Inclusion criteria
* ocular infection
Exclusion criteria
* Local antibiotics treatment
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Antibacterial effect of Urea Solution against Ocular Bacterial | 6 month | Samples were both inoculated on Muller Agar (Sigma-Aldrich) plates and directly examined under the microscope using Gram stained smears. Inoculated plates were incubated at 37oC for 48 hrs. Gram negative isolates were sub-cultured on MacConkey's, Eosin Methelyene Blue (EMB) and Cystine Lactose Electrolyte Deficient (CLED) agar plates (Sigma-Aldrich). Gram positive isolates were sub-cultured on Mannitol Salt agar plates (Sigma-Aldrich). |
Countries
Egypt
Outcome results
None listed