Periodontitis
Conditions
Keywords
Periodontitis, PPAR-γ, RXR-α, VDR
Brief summary
Objective: The present study aimed to determine the expressions of nuclear receptors PPAR (Peroxisome proliferative activator receptor)-γ, RXR (Retinoid X receptor)-α, and vitamin D receptor (VDR) in healthy volunteers and periodontitis patients with different grade involvement. Methods: Group-1; healthy individuals, Group-2; periodontitis patients-stage 3 grade B, (H-GradeB), Group-3; periodontitis patients-stage 3 grade C, (D-GradeC). Clinical parameters as plaque index (PI), gingival index (GI) and clinical attachment levels (CAL) were measured. Fibroblast and inflammatory cells, PPAR-γ, RXR-α, and VDR levels were determined in histological slides.
Interventions
DIAGNOSTIC_TESTGingiva Biopsy Sampling
Gingival samples of healthy individuals were collected by gingivectomy or crown lengthening procedure in the routine treatment protocol or before orthodontically indicated tooth extraction. Gingival samples of periodontitis patients were obtained during the scaling and root planning procedure. Posterior maxillary teeth (#4, #5, #6, and #7) were chosen for gingival sampling.
Sponsors
Tokat Gaziosmanpasa University
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Healthy volunteers
Yes
Inclusion criteria
* systemic health * no drug use * no previous periodontal treatment within six months * no tobacco use
Exclusion criteria
* pregnant or lactating women * patients used antibiotics in the last 6 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Inflammatory cell counts | During histological tissue processing | Connective tissues were evaluated, and inflammatory cell infiltration were counted in the H&E stained slides using a light microscope (Nikon Eclipse, E 600, Tokyo, Japan). For cell counting, connective tissue neighboring gingival epithelium was marked, and a cell counting frame of 10000 µm2 was marked. Inflammatory cells (neutrophil, lymphocyte, eosinophil, and macrophage cells) within the frame were counted. The measurements were performed from three different points, and the mean of these three measurements was recorded. |
| Fibroblasts counts | During histological tissue processing | For cell counting, connective tissue neighboring gingival epithelium was marked, and a cell counting frame of 10000 µm2 was marked. The fibroblast counts within the frame were counted. The measurements were performed from three different points, and the mean of these three measurements was recorded. |
| PPAR (Peroxisome proliferative activator receptor)-γ | During histological tissue processing | PPAR (Peroxisome proliferative activator receptor)-γ levels were determined via immunohistochemistry. |
| RXR (Retinoid X receptor)-α | During histological tissue processing | RXR (Retinoid X receptor)-α levels were determined via immunohistochemistry. |
| Vitamin D receptor (VDR) | During histological tissue processing | Vitamin D receptor (VDR) levels were determined via immunohistochemistry. |
Countries
Turkey (Türkiye)
Contacts
Primary ContactOzkan KARATAS
Outcome results
None listed