Insulin Resistance
Conditions
Keywords
INSULIN RESISTANCE
Brief summary
The study aims at the identification and the quantification of urinary branched-chain amino acids (BCAAs) by mass spectometry in healthy subjects and patients with insulin resistance and correlating urinary BCAAs with HOMA index. Blood and urine levels of BCAAs will be correlated with HOMA index to assess and quantify insulin resistance. Identification and quantification of urine BCAAs by an innovative method based on synthetic biology will also be performed. Correlations between mass spectometry and the innovative detection method of BCAAs will be searched.
Detailed description
Blood and urine samples will be collected in 330 persons to assess BCAAs levels and correlate them with HOMA index. Persons included will be: 110 persons with BMI\>27 and HOMA \>4 with no diabetes 110 persons with BMI\>27 and HOMA \< 4 with no diabetes 110 persons with BMI \< 27 and HOMA \<4 with no diabetes, including at least 50 men and 50 women, 30 aged 18-35, 30 aged 36-55, 30 aged 56-75. Urine BCAAs will be measured by both mass spectrometry and the innovative method based on synthetic biology.
Interventions
DIAGNOSTIC_TESTassessment of biomarkers of insulin resistance
blood and urine sample collection for assays of BCAAs
Sponsors
Centre National de la Recherche Scientifique, France
Sys2Diag, Mixt laboratory CNRS/Alcediag, Montpellier
University Hospital, Montpellier
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Intervention model description
Cohort of subjects with/without overweight and with/without insulin resistance
Eligibility
Sex/Gender
ALL
Age
18 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* no diabetes * according to studied subpopulations: BMI\<27 and HOMA \<4, BMI\>27 and HOMA\>4, BMI\>27 and HOMA\<4
Exclusion criteria
* pregnancy or lactation * active or suspected chronic infection * treatment affecting insulin sensitivity
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Correlation between urinary levels of BCAAs and HOMA index | through study completion, an average of two years and a half | Identification of urinary BCAAs as markers of insulin resistance |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Correlation between blood and urine BCAAs levels | through study completion, an average of two years and a half | Validation of urinary BCAAs as biomarkers of insulin resistance |
| Correlation between urine BCAAs levels measured by enzymatic method (artificial vesicles) and classic method | through study completion, an average of two years and a half | Measured by enzymatic method (artificial vesicles) and classic method (standard LC-MS/MS) |
| Correlation between urine BCAAs levels measured by enzymatic method (artificial vesicles) and HOMA index | through study completion, an average of two years and a half | Measured by enzymatic method (artificial vesicles) and HOMA index |
Countries
France
Outcome results
None listed