Colorectal Cancer
Conditions
Keywords
colorectal cancer, biomarkers, cancer risk, cancer prevention, ageing, diet, obesity, physical activity, colorectal adenoma
Brief summary
Worldwide, colorectal cancer is the 3rd most common cancer; risk increases with age and is modified by lifestyle factors notably diet, physical activity and obesity. The BORICC Follow-Up (BFU) Study is a 12+ year follow-up of participants recruited to the Biomarkers of Risk of Colon Cancer (BORICC) Study. This longitudinal study will investigate associations between ageing and lifestyle factors and a panel of molecular biomarkers linked with colorectal cancer risk.
Detailed description
The BORICC Follow-Up (BFU) Study builds on the findings from the BORICC Study where the investigators observed associations between age and nutritional factors, including selenium and folate, and biomarkers of colorectal health. The BFU Study will investigate the relationships between ageing (12+ years) and such biomarkers longitudinally. It is anticipated that the project will produce novel data on (i) changes in biomarkers of colorectal cancer risk with age and (ii) the effects of obesity and lifestyle factors on biomarkers of colorectal cancer risk. These biomarkers include differentially expressed proteins, DNA methylation markers and inflammation markers.
Interventions
None listed
Sponsors
Northumbria Healthcare NHS Foundation Trust
Newcastle University
Study design
Observational model
COHORT
Time perspective
OTHER
Eligibility
Sex/Gender
ALL
Healthy volunteers
Yes
Inclusion criteria
* Took part in the BORICC Study at baseline (recruited 2005/6)
Exclusion criteria
* Unable to travel to the hospital to attend study visit * Unable to provide informed written consent
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Faecal calprotectin concentrations (marker of local inflammation) | 12 years (on average) |
| Serum high-sensitivity C-Reactive Protein concentrations (marker of systemic inflammation) | 12 years (on average) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| microRNA expression in rectal mucosal biopsies | 12 years (on average) | Expression of microRNAs in rectal mucosal biopsies |
| Markers of mitochondrial function and structure in rectal mucosal biopsies | 12 years (on average) | Markers of mitochondrial function and structure in rectal mucosal biopsies such as oxidative phosphorylation proteins, namely complex I and IV |
| Parathyroid hormone in plasma | 12 years (on average) | — |
| 25-hydroxy vitamin D concentrations in serum | 12 years (on average) | — |
| Vitamin B12 concentrations in serum | 12 years (on average) | — |
| Folate concentrations in serum | 12 years (on average) | — |
| Triglycerides in plasma | 12 years (on average) | — |
| HDL cholesterol in plasma | 12 years (on average) | — |
| LDL cholesterol in plasma | 12 years (on average) | — |
| Total cholesterol in plasma | 12 years (on average) | — |
| Glucose in plasma | 12 years (on average) | — |
| HbA1c in whole blood | 12 years (on average) | — |
| Gut microbiota (analysed in stool samples) | 12 years (on average) | Abundance and diversity of the gut microbiota assessed in stool samples |
| Faecal short-chain fatty acid concentrations | 12 years (on average) | Concentrations and proportions of short-chain fatty acids e.g. acetate, propionate and butyrate |
| DNA methylation in rectal mucosal biopsies(% methylation) | 12 years (on average) | Target gene and global methylation (LINE-1) in rectal mucosal biopsies |
| Target gene expression in rectal mucosal biopsies | 12 years (on average) | Expression of genes related to inflammation and the WNT signalling pathway in rectal mucosal biopsies |
| Colonic crypt cell proliferative state | 12 years (on average) | Total number and distribution of proliferating cells in rectal mucosal crypts |
| Colonic crypt cell dimensions | 12 years (on average) | Height (length) and width rectal mucosal crypts |
Other
| Measure | Time frame | Description |
|---|---|---|
| Body weight in kg | 12 years (on average) | — |
| BMI in kg/m2 (calculated from height and weight) | 12 years (on average) | — |
| Height in cm | 12 years (on average) | — |
| Body fat percentage | 12 years (on average) | Measured using Tanita Bioimpedance scales |
| Habitual dietary intake assessed using Food Frequency Questionnaire | 12 years (on average) | — |
| Physical activity levels assessed using Lifestyle Questionnaire | 12 years (on average) | — |
| Physical activity levels assessed using accelerometer | 12 years (on average) | — |
| Sedentary behaviour assessed using Lifestyle Questionnaire | 12 years (on average) | — |
| Heel bone density using Achilles heel ultrasound device | 12 years (on average) | — |
| Timed-up and go test time | 12 years (on average) | — |
| Hand grip strength using dynamometer | 12 years (on average) | — |
Countries
United Kingdom
Outcome results
None listed