Postprandial Glucose and Insulin Response
Conditions
Brief summary
Individuals with low copy numbers of salivary amylase gene have lower salivary amylase levels and might therefore have decreased capacity to metabolize starch into glucose. We will in a randomized crossover meal study examine the postprandial response of high-starch meals in individuals with either low or high copy number of the salivary amylase gene.
Interventions
OTHER40 grams of starch
40 grams of starch in form of white bread to be consumed within 15 minutes
OTHER80 grams of starch
80 grams of starch in form of white bread to be consumed within 15 minutes
Sponsors
University of Copenhagen
Lund University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
TRIPLE (Subject, Caregiver, Outcomes Assessor)
Masking description
Genotype were blinded for the participants and all research staff except for the principal investigator. Amount of bread was not blinded.
Intervention model description
Participants with low and high copy numbers of AMY1 were randomized to either 40 or 80 grams of starch in a crossover design.
Eligibility
Sex/Gender
ALL
Age
25 Years to 70 Years
Healthy volunteers
No
Inclusion criteria
* Participant in the Malmö Offspring Study * Genotyped for AMY1 copy number variation and selected for having either low (2-4) or high (10 or above) copy number of AMY1 * Gender: both males and females * Completed information on dietary habits
Exclusion criteria
* Allergic to wheat, gluten intolerant or on a low-carbohydrate diet * Inability to eat up to 4 slices of white bread in 15 minutes * Use of medication known to influence glucose metabolism, appetite or saliva flow * Diagnosed diabetes or high fasting plasma glucose (above 6.1 mmol/L) * Smoking
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Postprandial glucose | AUC (0, 7, 15, 30, 45, 60, 90, 120 minutes) | Postprandial glucose concentration assessed as incremental area under curve over 120 minutes (0, 7, 15, 30, 45, 60 ,90, 120 minutes) |
| Postprandial insulin | AUC (0, 7, 15, 30, 45, 60, 90, 120 minutes) | postprandial insulin concentration assessed as incremental area under curve over 120 minutes (0, 7, 15, 30, 45, 60 ,90, 120 minutes) |
| Preabsorptive insulin release | 7 minutes | Insulin concentration at 7 minutes |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| subjective satiety | 0, 15, 30, 45, 60, 90, 120 minutes | Visual analog scales (VAS) to evaluate hunger and satiety |
| salivary amylase activity | 0 minutes | Salivary amylase activity will be measured by ELISA kits |
Other
| Measure | Time frame | Description |
|---|---|---|
| weight | 0 minutes | weight will be measured |
Countries
Sweden
Outcome results
None listed