Skin Rejuvenation, Lipofilling
Conditions
Keywords
adipose tissue derived stem cells, lipofilling
Brief summary
this research is to study the effect of Adipose Derived Stem Cells on Survival of Fat as Filler
Detailed description
Skin aging is a complex biological process.The physiological changes associated with aging of the skin are manifested in xerosis, dramatic loss of skin elasticity due to damage to collagen and elastin fibers; as well as barrier function, modification of rhytides and deficiencies in the regenerative property of the skin. All of which ultimately result in thinning of the skin, malar fat atrophy and pigmentary changes. Autologous fat grafting or lipo injection containing stromal vascular fraction (SVF) acts like ideal soft tissue filler for facial filling and rejuvenation. It leads to progressive improvement of the skin texture, elasticity, and color over a few months, therefore adipose tissue seems to be not only a simple filler but also a dynamic filler with two types of different and supplementary effects, the volumetric effect and the regenerative effect.Cell assisted lipotransfer (CAL) is a technique that combines aspirated fat with concentrated ADSCs in the stromal vascular fraction (SVF) of the lipoaspirate. This technique could enhance the survival rate of the transplanted fat and leads to better cosmetic improvement
Interventions
PROCEDURElipofilling
Under local anesthesia using strict aseptic technique, a small incision will be done in the lateral aspect of the thigh or lower abdomen, through which the infiltration cannula will be introduced to inject the local anesthetic solution using the wet technique. This will be followed 15 minutes later by lipo-aspiration of 75 ml fat ) using a blunt tipped cannula under the negative suction pressure of a 60 ml syringe. 50 ml is used for preparation of microfat. then with blunt cannula,subcutaneous injection of the required volume of microfat is placed into temporal region.
PROCEDURElipofilling enriched with adipose tissue derived stem cells
Under local anesthesia using strict aseptic technique, a small incision will be done in the lateral aspect of the thigh or lower abdomen, through which the infiltration cannula will be introduced to inject the local anesthetic solution using the wet technique. This will be followed 15 minutes later by lipo-aspiration of 75 ml fat using a blunt tipped cannula under the negative suction pressure of a 60 ml syringe. 50 ml is used for preparation of microfat. 25 ml is used for preparation of autologous adipose tissue derived stem cells (At-ADSCs) using enzymatic digestion and differential centrifugation in the Center of Excellence for Research in Regenerative Medicine and its Application (CERRMA), Alexandria Faculty of Medicine. Subcutaneous injection of the required volume of microfat combined with stromal vascular fraction containing adipose tissue derived stem cells, is placed into temporal region.
Sponsors
Alexandria University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Intervention model description
split face
Eligibility
Sex/Gender
FEMALE
Age
35 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
1. Clinically diagnosed facial skin aging. 2. Glogau photoaging score II and III. 3. Body mass index ≥20 with adequate abdominal or other subcutaneous adipose tissue accessible for lipoaspiration.
Exclusion criteria
1. History of keloid formation. 2. Any coincidental chronic illness (e.g. metabolic, autoimmune or endocrinal) or malignancy. 3. Any bleeding or coagulation disorder or recent use of anticoagulant therapy. 4. Active infection. 5. History of any previous aesthetic procedure on the face within the past 6 months. 6. History of intake of anti-aging systemic or topical medications within the previous 3 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Assessment using hollowness severity rating scale :0 : no visible hollowness, 1: mild Hollowness, 2 : moderate Hollowness, 3: severe Hollowness | 3 months, 6 months | serial photography for assessment using hollowness severity rating scale :0 : no visible hollowness, 1: mild Hollowness, 2 : moderate Hollowness, 3: severe Hollowness |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Measurement of hypodermal thickness in mm using UBM (Ultrasound Bio microscopy) on both temple regions of the head | 3 months, 6 months | Measurement of hypodermal thickness in mm using UBM (Ultrasound Bio microscopy) on both temple regions of the head |
| Measurement of dermal thickness in mm using UBM (Ultrasound Bio microscopy) on both temple regions of the head Measurement of dermal thickness in mm using UBM (Ultrasound Bio microscopy) on both temple regions of the head | 3 months, 6 months | Measurement of dermal thickness in mm using UBM (Ultrasound Bio microscopy) on both temple regions of the head |
Countries
Egypt
Outcome results
None listed