Infertility
Conditions
Brief summary
The mail aim of the present study is to use molecular methods to analyze the ability of human preimplantation embryos to repair their DNA
Detailed description
The main aim of the present study is to investigate the DNA repair pathways of preimplantation embryos, examining the events that take place following induction of a DSB at a unique targeted site. The investigators aim to address questions concerning the functionality of the DNA repair mechanism(s) in developing embryos, before and after genome activation.
Interventions
GENETICdouble strand break
an induction of a double strand break will be made in the embryos generated for this research and the repair mechanism observed
Sponsors
Reproductive Medicine Associates of New Jersey
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
21 Years to 42 Years
Inclusion criteria
* gamete donors: be at least 21 years of age, in good health and eligible to donate their games through the standard gamete donation programs * IVF couples: any couples proceeding with a routine IVF cycle
Exclusion criteria
* less than 21 years of age * inability to provide informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| repair of double strand break in DNA | up to 12 days | targeted deep sequencing to identify the presence of the double strand break in the DNA and subsequent repair ability of the embryo |
Countries
United States
Outcome results
None listed