Cutibacterium Acnes in Bone and Joint Infections

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT03950063

Acronym

CaBJI

Enrollment

Registered

2019-05-15

Start date

2019-06-15

Completion date

2021-06-15

Last updated

2019-05-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Cutibacterium Acnes, Prosthesis and Bone Infections

Keywords

Cutibacterium acnes Prosthesis, Bone Infections

Brief summary

Prosthetic Joint Infections (PJIs) are increasing with the use of orthopedic devices on an ageing population. Cutibacterium acnes is a commensal organism that plays an important role in the ecosystem healthy human skin, yet this species is also recognized as a pathogen in foreign body infection: endocarditis, prostatitis and specifically in PJIs. C. acnes is able to escape the immune system. This phenomenon could reflect two bacterial behavior: the bacterial internalization by host cells and the biofilm formation.

Detailed description

The aim of the study is to evaluate specific pathogenicity factors (ability to internalize bone cells and ability to form a biofilm) of bacteria derived from clinical strains of C. acnes isolated from bone infections on orthopedic material.

Interventions

None listed

Study design

Observational model

CASE_ONLY

Time perspective

RETROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Inclusion criteria

* bone infection with C. acnes, * isolation of the strain, * presence of a single species, * conservation of a viable strain, * patients over 18 years

Design outcomes

Primary

Measure	Time frame	Description
Internalization capacity of C. acnes strains	Day 0	C. acnes strains will be cultured for 5 days in rich medium at 37°C in anaerobic conditions. Then, the bacteria will be co cultured with bone cells (osteoblast) and bacteria will be internalized by the bone cells. After killing bacteria stayed outside of the cells with an antibiotic and lysis of the cells, the number of bacteria internalized will be counted on agar plate.
Biofilm capacity of C. acnes strains	Day 0	C. acnes strains will be cultured for 5 days in rich medium at 37°C in anaerobic conditions. Then, the biofilm biomass will be evaluated by Crystal violet staining. The amount of biofilm will be evaluated by absorbance (optic density by spectrophotometry).

Countries

France

Contacts

Primary ContactXavier OHL

xohl@chu-reims.fr03 26 78 32 72

Outcome results