Ocular Toxoplasmosis
Conditions
Brief summary
Ocular toxoplasmosis (OT) is a common cause of posterior uveitis worldwide. The diagnosis of OT is based on clinical findings, but in most cases, laboratory tests are required to confirm the etiology, especially when other diseases are suspected. The aim of this study was to evaluate which methods, between the Goldmann-Witmer coefficient (GWC) and immunoblotting (IB) with both IgG and IgA, in aqueous humour (AH) samples, can be the most sensitive to diagnose OT, in current practice, especially in the first three weeks.
Detailed description
Goldmann-Witmer coefficient (GWC): compares the levels of intraocular antibody (igG) production to that serum, as measured by ELISA. GWC = (Level of specific IgG in aqueous humour/level of specific IgG in serum)/(Total IgG in aqueous humour/Total IgG in serum) 0.5 to 2: No intraocular antibody production, 2 to 4: Suggestive of intraocular antibody production, \> 4: Diagnostic of intraocular antibody production to a specific microbial pathogen
Interventions
PROCEDUREAqueous humour sampling
Aqueous humour sampling with Goldman-Witmer coefficient and immunoblotting
Sponsors
Hospices Civils de Lyon
Study design
Observational model
CASE_CONTROL
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* Patients who had underwent aqueous humor and serum sample for ocular toxoplasmosis diagnosis
Exclusion criteria
* Immunocompromised patients
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Comparison of Goldmann-Witmer coefficient and immunoblotting results | Assessment of each test result at the inclusion of the patient | All tests realized in patients were performed before their repartition in the two groups as well as the biologist could not know the status of the patient. Sensitivity and specificity were calculated for each test. |
Countries
France
Outcome results
None listed