Malignant Breast Tissue Neoplasm
Conditions
Keywords
Beetroot juice, Magnetic Resonance Imaging, Breast cancer
Brief summary
Breast cancer is the most prevalent cancer affecting women, with 1 in 8 women in the UK developing breast cancer in their life time. Chemotherapy drugs, currently used for locally advanced breast cancers, are associated with side effects while dietary supplements have complex effects with a relatively small effect size. Breast tumours have different metabolism compared to healthy tissue, including elevated lactate production by aerobic glycolysis (AG), an underpinning feature of metabolism in breast cancer cells. Dietary nitrate, contained in leafy green vegetables and beetroot, has been shown to improve energy efficiency in exercising skeletal muscle, positioning itself as a disruptor of AG. The purpose of this study is to examine if dietary nitrate can disrupt AG and as a result to halt or even reverse tumour progression and survival. This study will look at scans of breast tumours using magnetic resonance imaging (MRI). Changes to tumour related biochemical substances will be measured by advanced magnetic resonance spectroscopy and changes to tissue structure will be measured by advanced diffusion MRI techniques. In this study, 16 patients undergoing surgery will be recruited for two MRI scans following a 5 day intervention programme.
Detailed description
In this longitudinal study, the investigators propose to examine the changes in lipid composition and microstructure in breast tumours at baseline and post dietary nitrate intervention through the applications of novel non-invasive magnetic resonance spectroscopy (MRS) methods and diffusion and lipid profiling MR imaging methods in patients. The investigators hypothesise that dietary nitrate disrupts tumour progression in breast cancer. 1. Is there a significant difference in lipid composition in the tumour measured by MRS between baseline and post dietary nitrate intervention? 2. Is there a significant difference in lipid composition in the whole breast tissue surrounding tumour measured by MRI between baseline and post dietary nitrate intervention? 3. Is there a significant difference in tissue microstructure measured by MRI between baseline and post dietary nitrate intervention in the breast tissue? To assess the effect of dietary nitrate on tumour progression, paired t-tests will be performed on lipid composition measured from tumour between baseline and post intervention. To assess the effect of dietary nitrate on tumour progression, paired t-tests will be performed on lipid composition in the whole breast tissue surrounding tumour between baseline and post intervention. To assess the effect of dietary nitrate on tissue microstructure paired t-tests will be performed on water displacement measured from tumour between baseline and post intervention.
Interventions
DIETARY_SUPPLEMENTBeetroot juice
The patient will follow a 5 day dietary nitrate intervention programme, taking 3 doses of 7cl (0.4 g nitrate per dose) concentrated beetroot juice (SPORT shot, James White Drinks, Suffolk, U.K.) a day.
Sponsors
University of Aberdeen
NHS Grampian
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Masking description
Single group with no masking
Intervention model description
Single Group: trials with a single arm
Eligibility
Sex/Gender
FEMALE
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients with pathologically confirmed invasive ductal carcinoma (Grade 2 or 3) * Undergoing wide local excision surgery or mastectomy
Exclusion criteria
* Started hormone or chemotherapy treatment before recruitment. * Undergoing treatment for concurrent cancer diagnosis. * Taking antibiotics, proton pump or vegetable dietary supplement.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Baseline: Lipid composition in tumour | Scan one day before intervention (Day 0) | Lipid peak volume with units of ratio |
| Post intervention: Lipid composition in tumour | Scan one day after completion of intervention (Day 6) | Lipid peak volume ratio with units of ratio |
| Baseline: Lipid composition in whole breast tissue surrounding tumour | Scan one day before intervention (Day 0) | fat fraction with units of % |
| Post intervention: Lipid composition in whole breast tissue surrounding tumour | Scan one day after completion of intervention (Day 6) | fat fraction with units of % |
| Baseline: Water displacement in tumour | Scan one day before intervention (Day 0) | Water displacement with units of micrometer |
| Post intervention: Water displacement in tumour | Scan one day after completion of intervention (Day 6) | Water displacement with units of micrometer |
Countries
United Kingdom
Contacts
STUDY_CHAIRJiabao He, PhD
University of Aberdeen
PRINCIPAL_INVESTIGATORVasiliki Mallikourti, MSc
University of Aberdeen
Outcome results
None listed