Healthy
Conditions
Brief summary
The present study will seek the quantify the simultaneous muscle protein synthesis and breakdown response with and without amino acid provision in humans following 2 days of immobilisation.
Interventions
OTHERPostabsorptive
Saline will be infused to mimic postabsorptive circulating amino acid concentrations
OTHERPostprandial
An amino acid infusate will be administered to mimic postprandial circulating amino acid concentrations
PROCEDUREImmobilisation
One leg will undergo 2 days immobilisation prior to the test day
Sponsors
University of Exeter
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
MALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Male * 18-40 years of age * Body mass index between 18.5 and 30
Exclusion criteria
* Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes) * Any diagnosed cardiovascular disease (e.g. deep vein thrombosis) or hypertension * Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism) * A personal or family history of epilepsy, seizures or schizophrenia * Any known disorders in muscle metabolism * Regular use of nutritional supplements * Allergy to lidocaine * Allergy to one or multiple amino acids * Recent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture) as these could be exacerbated by the intervention e.g. unilateral leg immobilisation, or mean the participant is unable to use crutches. * Having received or ingested a stable isotope tracer in the past
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Muscle protein breakdown rate | 1 hour | Muscle protein breakdown rate (FBR, measured in %/h) during 1 hour of saline or amino acid infusion |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Muscle protein breakdown rate | 3 hours | Muscle protein breakdown rate (FBR, measured in %/h) during 3 hours of saline or amino acid infusion |
| Muscle protein synthesis rate | 3 hours | Muscle protein synthesis rate (FSR, measured in %/h) during 3 hours of saline or amino acid infusion measured using a D5 phenylalanine infusion |
Countries
United Kingdom
Outcome results
None listed