Atherosclerosis Cerebral, Microbiome, Stroke, Stroke, Acute, Stroke, Ischemic
Conditions
Keywords
Stroke, Ischemic stroke, microbiome, atherosclerotic
Brief summary
This study is to find out the significance of gut-microbiota in acute stroke patients, including their neurological, radiological outcomes as well as their stroke mechanisms.
Detailed description
Patients who suffered from acute stroke and in hospitalization in Prince of Wales Hospital will be recruited in the study. After the informed consent, their first bowel opening will be collected for storage. They will also receive assessment by the stroke team at 3 and 6 months to determine their outcomes (NIHSS and mRS). Clinically and radiologically parameters including their degree of disability, imaging findings will also be collected to determine whether there are any correlations with the stool microbiota composition against matched individuals.
Interventions
None listed
Sponsors
Chinese University of Hong Kong
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
1. Diagnosed as acute ischaemic stroke 2. Aged 18 or above Chinese 3. Radiological evidence of acute ischaemic stroke by Computed tomography (CT) or magnetic resonance imaging (MRI) brain.
Exclusion criteria
1. Patient with symptoms and signs suggestive of alternative diagnoses, 2. Evidence of intracerebral haemorrhage, 3. Absence of DWI evidence of acute ischaemic infarct, 4. Pregnancy, 5. Evidence of gastrointestinal infection/ inflammation/ obstruction 6. History of partial or total resection of small or large bowel, as well as gut re-anastomosis, 7. Use of antibiotics within 2 weeks prior to symptoms onset, 8. Gastrointestinal malignancy 9. Any hospitalization within 3 months before recruitment 10. Institutionalized patients
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The composition of gut-microbiota contributing neurological outcome between groups | 31 Dec, 2020 | Gene sequencing of the 16S rRNA on the stool samples are performed to identify the microbes down to genus level, as well as the microbrobiota diversity and relative abundance. And stroke etiology classified by TOAST Classification and disability indices (NIHSS \& mRS at baseline, 3 month \& 6 month) will be recorded. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| The composition of gut-mircobiota contributing radiological finding between groups | 31 Dec, 2020 | Gene sequencing of the 16S rRNA on the stool samples are performed to identify the microbes down to genus level, as well as the microbrobiota diversity and relative abundance. All patients who received a plain CT brain \& MRI for the diagnosis of stroke will be reviewed. Infarct volume in DWI, MR angiography abnormalities, presence of microbleed and/or haemorrhagic transformation will be documented. |
Countries
Hong Kong
Contacts
PRINCIPAL_INVESTIGATORYiu Ming Bonaventure IP, MRCP
Chinese University of Hong Kong
Outcome results
None listed