Pregnancy Related
Conditions
Keywords
Transcriptome, endometrial receptivity
Brief summary
The aim of this study is to correlate and identify the biochemical markers which may reflect WOI. Blood samples will be collected and endometrial biopsy will be performed at particular time points.
Detailed description
Endometrial receptivity in the midluteal phase of the menstrual cycle, known as the window of implantation (WOI), occurs only for a very short period of time. However, so far, there is no reliable clinical and biochemical indicators of WOI. Therefore, this study is to correlate and identify the biochemical markers which may reflect WOI. participants will be asked to be: 1. performed endometrial biopsy 2. taken blood for a few time points (e.g. pre-conception and being pregnant) and to compare different kinds of biochemical markers.
Interventions
PROCEDUREendometrial biopsy and blood sampling
patient will be performed endometrial biopsy and collected blood samples at particular time points
Sponsors
Chinese University of Hong Kong
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 40 Years
Inclusion criteria
Age 20-40 years.
Exclusion criteria
* Uncorrected uterine anomalies, such as septate or bicornuate uterus, fibroids (submucous/intramural). * Intrauterine adhesions. * Adenomyosis.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The change of the transcriptome profiling in the peripheral blood | From the day of endometrial biopsy through study completion, an average of 2 months | RNA per sample will be prepared into two portions. One is for RNA sequencing and the other is for small RNA sequencing. The next step is hydrolysis of RNA into 200-300 nucleotides prior to reverse transcription. When sequencing RNA other than mRNA the library preparation is modified. The cellular RNA is selected based on the desired size range. For miRNA, the RNA is isolated through size selection. Once isolated, linkers are added to the 3' and 5' end then purified. The final step is cDNA generation through reverse transcription. |
| The change of the immune markers in the peripheral blood | From the day of endometrial biopsy through study completion, an average of 2 months | Immune markers including cytokines such as leukemia inhibitory factor, macrophage colony stimulating factor, interleukin - 1 beta, transforming growth factor beta-1, Tim-3, galectin-9 and other markers considered to play a role in implantation will also be measured. |
Countries
Hong Kong
Contacts
Primary ContactHiu Tan Margaret Lee
Backup ContactPui Wah Jacqueline Chung, MBBS
Outcome results
None listed