Infertility
Conditions
Brief summary
High follicular fluid amyloid A level is associated with reduced pregnancy rate. The aim of this study was to find an association between serum level of amyloid A and unexplained infertility.
Interventions
For determination of SAA level, a 5-ml sample of venous blood will be taken from each participant under aseptic conditions, and centrifuged at 2500 g for 15 minutes at 4°C c. separated into serum aliquots,and stored frozen at -80°C until analysis of SAA ,which will be done by a double-antibody sandwich Enzyme Linked Immunosorbent Assay (ELISA) kits supplied by cusabio,USA according to manufacturers instruction .Each sample was tested in duplicate and the average will be taken. A cut off level of 30 mg/L SAA level will be chosen to reflect a real increase in its level
Sponsors
Ain Shams Maternity Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
No minimum to 40 Years
Healthy volunteers
Yes
Inclusion criteria
1. women with unexplained infertility (unprotected, regular intercourse for at least 2 years). 2. Nulligravida ,Regular mensturation. 3. Age less than 40 years . 4. Normal parameters (in the study group): * hormonal profile (day 2 FSH,LH), midluteal phase progesterone, Prolactin, TSH * transvaginal ultrasound * hysterosalpingography * detailed semen analysis
Exclusion criteria
1. Age above 40 2. Smoking females 3. Obesity (BMI\>30) 4. Women with any systemic or medical diseases leads to elevation of SAA as: 1. Systemic lupus erythematosis 2. Rheumatoid arthritis 3. Sarcoidosis 4. Diabetes Mellitus 5. Using hormonal contraception (for control group)
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Difference between SAA level between cases and control. | 1 day (once) |
Secondary
| Measure | Time frame |
|---|---|
| Correlation between SAA level with other parameters in the hormonal profile (e.g., FSH, LH, E2). | 1 day (once) |
Countries
Egypt
Outcome results
None listed