Gestational Weight Gain, Microbiota, Pregnancy
Conditions
Brief summary
A prospective, multicenter, observational cohort study including about 550 mother-infant pairs in Beijing will be conducted to evaluate the association between mothers' gestational weight gain and the gut microbiota of them and their infants.
Detailed description
Purpose To evaluate whether mothers' gestational weight gain is associated with differences in maternal and their infants gut microbiota characteristics. Methods and analysis This is a prospective, multicenter, observational cohort study including about 550 mother-infant pairs from PUMCH and other 3 hospitals in Beijing. After signing consent form, every participant will bring home a weighting scale for checking and record the weight every week from they enrolled to a year postpartum. The blood samples, fecal samples, vaginal swabs and oral swabs of the mothers will be collected at first, second, third trimester and postpartum 42 days. The infants' fecal samples will be collected at 0\ 3, 14, 42 days and 3, 6, 9, 12 months after born. Questionnaire for mothers (diet, exercise, antibiotic and probiotic usage, oral hygiene habits etc.) and their infants (delivery mode, feeding pattern, antibiotic and probiotic usage etc.), as well as the medical records will be collected along with the sample collecting. Bio-specimen will be tested by 16S rRNA gene sequencing. Logistical regression and covariance analysis will be used to determine the relationships between the demographic data, gestational weight gain and microbiota composition.
Interventions
None listed
Sponsors
Peking Union Medical College Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Ensure a regular prenatal check in the clinical centers. * 20-45 years old. * 8-13 gestational weeks. * Singleton. * Fully understand and voluntarily sign the informed consent.
Exclusion criteria
* IUI or IVF-ET pregnancy. * Smoking or drinking. * Antibiotics exposure within a month. * With metabolic syndrome, hypertension, diabetes or dyslipidemia before pregnancy. * With digestive disease, uncontrolled thyroid disease.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| maternal gut microbiota, vaginal microbiota, oral microbiota | From enrollment to 42 days postpartum | The outcome will be measured at first, second and third trimester, as well as 42 days postpartum. |
| gut microbiota infants | From delivery to 1 year postpartum. | The outcome will be measured at seven specific time points after the babies delivered. |
Countries
China
Contacts
Primary ContactLiangkun Ma, doctor
Backup ContactYing Tian, master
Outcome results
None listed