Type1 Diabetes Mellitus
Conditions
Keywords
diabetes mellitus, children, microbiome
Brief summary
Aims: To profile the oral microbiome of children with type 1 diabetes mellitus, and to compare it to healthy children while taking into account other aspects of the oral environment. Research: exploring the differences between oral microflora of children with type 1 diabetes mellitus and control healthy children using salivary microbiome. The investigators will furthermore intend to analyze the differences of salivary flow rate, pH, glucose, calcium, inorganic phosphate and urea between the two groups.
Detailed description
Saliva from 50 children ages 5 to 15 years old who had been diagnosed with type 1 diabetes mellitus and are followed at the Pediatric Endocrinology Clinic, Hadassah University Hospital Mt. Scopus and Ein Kerem, Jerusalem, Isreal, will be compared to 50 healthy children, preferably their siblings, matched in age and gender. Each patient or their legal guardian will sign an informed consent to participate in the study. The investigators will request a permission to access the medical records of the patients. Data will be collected from patient medical history. The investigators will measure salivary flow rate, pH and glucose, salivary Calcium, inorganic phosphate and urea and perform microbiome analysis and 16S rRNA gene-based analysis comparing children with diabetes to healthy children.
Interventions
DIAGNOSTIC_TESTMicrobiome analysis and 16S rRNA gene-based analysis
microbial DNA is extracted from the saliva and 16s library is prepared for sequencing.
Sponsors
Hadassah Medical Organization
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
5 Years to 15 Years
Inclusion criteria
• Children with type 1 diabetes mellitus
Exclusion criteria
* Children Unable to give saliva sample * Children On antibacterial medicaments
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| profile the oral bacteria related to caries and periodontal disease of children with type 1 diabetes mellitus | 1 year | Profile salivary microbiota in diabetic children followed by bioinformatics analyses and combining the data with the alteration in caries index, medications, state of the disease and salivary components to give a list of specific bacteria that are different in the diabetic child from healthy population. |
Countries
Israel
Outcome results
None listed