Cataract
Conditions
Brief summary
To study the pathological mechanism of cataract by genomics, transcriptomics, and proteomics.
Detailed description
Tissue samples and aqueous humor samples will be collected from each individual patients by cataract surgery. After sample treatment, different techniques will be applied for analysis. And bioinformatics approach is applied for data treatment and analysis.The data of genomics, transcriptomics, and proteomics will be analyzed for study of pathological mechanism of cataract.
Interventions
PROCEDUREphacoemulsification
Sponsors
Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
5 Years to 94 Years
Healthy volunteers
No
Inclusion criteria
* Patients with the explicit diagnosis of cataract (including senile cataract, congenital cataract, complicated cataract, metabolic cataract, traumatic cataract, etc.) in the operated eye are willing to undergo cataract surgery.
Exclusion criteria
* Patients whose cataracts need not be operated * Patients with any contraindications to cataract surgery * Patients are unwilling to undergo cataract surgery
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Characterization of miRNA in micro-scale human aqueous humor | 12 month | method development for miRNA detection in aqueous humor |
| Favored amplification recovery via protection-chromatin immunoprecipitation-Seq of human eye tissue samples | 24 month | low-cell-number epigenome profiling for study of cataract |
| Study of cataract by genomics, transcriptomics, and proteomics approaches | 36 month | detect histone H3 lysine 4 trimethylation (H3K4me3) of aqueous humor of cataract patients by genomics, transcriptomics, and proteomics methods |
Countries
China
Contacts
Primary ContactHaidong Zou
Backup ContactBijun Zhu
Outcome results
None listed