Out-Of-Hospital Cardiac Arrest After Initial Successful Resuscitation
Conditions
Keywords
Transcriptome, messenger ribonucleic acid (mRNA), sequencing, biomarker, cardiac arrest, prognostication, successful resuscitation
Brief summary
The purpose of this study is to determine whether the blood transcriptome of patients resuscitated after out-of-hospital could be an early predictor of the neurological outcome.
Detailed description
Assessing the prognosis of the patients resuscitated after out-of-hospital cardiac arrest (OHCA) is still highly challenging. Beyond usual neurological markers, experimental and clinical studies have also shown that blood levels of several cytokines or miRNA could be significantly different after cardiac arrest in subjects prematurely died as compared to survivors with a good neurological recovery. Here the investigators propose to investigate a screening approach assessing simultaneously multiple targets of the post-cardiac arrest syndrome through analysis of the whole transcriptome of patients after OHCA. For this purpose, the investigators will collect blood samples from 60 patients after admission at hospital and then 1 and 3 days later. The Pittsburgh Cerebral Performance Category of each patient will be evaluated for each patient at day 60 after cardiac arrest. At the end of the collection period, the blood samples will be prepared for whole genome analysis and RNA sequencing. The primary analysis of the investigators will be the comparison of the transcriptome at day 0 after cardiac arrest in patients reaching Cerebral Performance Category (CPC) 1 at day 60 as compared to all other patients.
Interventions
BIOLOGICALblood sampling
Sampling of 15 ml of blood during a blood test at Day 0, Day 1 and Day 3
Sponsors
National Research Agency, France
University of Paris 5 - Rene Descartes
Institut National de la Santé Et de la Recherche Médicale, France
Paris 12 Val de Marne University
Ecole Nationale Vétérinaire d'Alfort
URC-CIC Paris Descartes Necker Cochin
Assistance Publique - Hôpitaux de Paris
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
* Age between 18 and 80 years * Delay between patients collapse and resumption of spontaneous circulation (ROSC) \< 60 minutes * Comatose patients ROSC with Glasgow score \< 7 * Patient already registered in the social security system * Consent of a family member, if able to understand in the emergency context, or attestation of emergency situation with a follow-up information notice
Exclusion criteria
* Cardiac arrest supposed to be provoked by trauma or sepsis
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Early Transcriptome at hospital admission | Day 0 | Blood samples will be collected for each patient early after admission at hospital and blood whole transcriptome will be assessed by RNA sequencing |
| Pittsburgh Cerebral Performance Category at Day 60 | Day 60 | Pittsburgh Cerebral Performance Category will be evaluated for each patient at day 60 |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Pittsburgh Cerebral Performance Category at Day 28 | day 28 | Pittsburgh Cerebral Performance Category will be evaluated for each patient at day 28 |
| Transcriptome at day 1 | Day 1 | Blood samples will be collected for each patient at day 1 and blood whole transcriptome will be assessed by RNA sequencing |
| Number of participants with death at day 60 | day 60 | — |
| Number of participants with death at day 28 | day 28 | — |
| Transcriptome at day 3 | Day 3 | Blood samples will be collected for each patient at day 3 and blood whole transcriptome will be assessed by RNA sequencing |
Countries
France
Outcome results
None listed