Healthy
Conditions
Keywords
metabolomics, proteomics, PUFA, nutrition, healthy people aged 18-50
Brief summary
Analysis of the effect of dietary intervention based on increased polyunsaturated fatty acid intake on health. Specifically changes in blood lipid profile, as well as proteomic and metabolomic profiles of healthy adults will be analyzed.
Interventions
OTHERThe high PUFA diet group
Assumptions of nutritional intervention: in a randomized manner volunteers will be assigned to one of two groups: a control group which will receive general nutritional recommendations, based on the Polish nutrition pyramid, and the intervention group which will receive nutritional recommendations, based on the Polish nutrition pyramid and will consume at least 4 tablespoons of olive oil or 30 grams of nuts every day for 12 weeks.
Sponsors
Poznan University of Life Sciences
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* good health, * proper body weight * not meeting the needs for polyunsaturated fatty acids
Exclusion criteria
* increased body weight, * limited communication to an extent that makes it impossible to carry out a food interview, * eating disorders (according to food history), * metabolic diseases, * pregnancy, * lactation, * taking drugs that influence the metabolism of lipids or carbohydrates
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Nutrient intake | 12 weeks | Nutrient intake (3-day food records) |
| Concentrations of lipoproteins and glucose | 12 weeks | Serum concentrations of lipoproteins and glucose at baseline and after the intervention \[mg/dl\] |
| Proteomic analysis of plasma | 12 weeks | The aim is to identify biomarkers in the blood and to determine the differences between people from the control and the intervention group |
| Metabolite concentrations in plasma | 12 weeks | Metabolite concentrations in plasma samples are measured by high resolution mass spectrometry at baseline and after the intervention |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Body mass | 12 weeks | body mass at baseline and after the intervention |
| hip circumference | 12 weeks | hip circumference at baseline and after the intervention |
| Height | 12 weeks | height |
| Percentage body fat | 12 weeks | percentage body fat at baseline and after the intervention |
| Waist circumference | 12 weeks | waist circumference at baseline and after the intervention |
Countries
Poland
Contacts
Primary ContactJoanna Ciborska, Ph.D
Backup ContactAgata Chmurzynska, Ph.D
Outcome results
None listed