Abnormality, Chromosome Abnormality
Conditions
Keywords
Diagnosis, Reproduction, Chromosomal Endowment
Brief summary
This study aims to validate a non-invasive method of chromosomal screening (NICS), based on the Multiple Annealing and Looping Based Amplification Cycles- Next-generation Sequencing (MALBAC-NGS) technology, in order to determine the chromosomal endowment of a blastocyst from the DNA of the embryonic culture medium. The chromosomal status of the embryo from an embryo biopsy of trophoectoderm will be established (usual clinical practice), the chromosomal endowment from the DNA of the embryonic culture medium will be determined, and the results using the NICS and the conventional invasive method (Preimplantation Genetic Testing for Aneuploidy \[PGT-A\]) will be compared.
Interventions
DIAGNOSTIC_TESTNoninvasive chromosome screening
Screening based on MALBAC-NGS technology
Sponsors
Instituto Bernabeu
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
MALE
Age
19 Years to 49 Years
Inclusion criteria
* Treatment by oocyte donation or own oocyte nd preimplantation genetic screening of aneuploidies (PGT-A). * Embryonic cryopreservation. * Informed consent signature
Exclusion criteria
* Embryo transfer without embryo freezing * Embryonic block. * Bad embryo quality.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Presence of Mosaicism | At Day 5-6 after FIV | Presence of mosaicism |
| Quality value of NICS DLRS | At Day 5-6 after FIV | Quality value of noninvasive chromosome screening (NICS) derivative Log Ratio Spread (DLRS) |
Countries
Spain
Outcome results
None listed