Healthy
Conditions
Keywords
High-fat diet, Insulin, Glucose, Muscle, eNOS, Isotopes
Brief summary
This study determined the effect of 7 days of high-fat overfeeding on whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function in 15 healthy young individuals.
Detailed description
Fifteen healthy, young individuals underwent metabolic testing before and after 7 days of high-fat overfeeding. We determined whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function. Stable isotope tracers were used to assess the rate of appearance of glucose from the gut, liver glucose output and whole-body glucose disposal. Insulin signalling was determined by Western blotting of skeletal muscle tissue. Microvascular function was assessed through the use of quantitative immunofluorescence microscopy. These measurements were performed both postabsorptively and following the ingestion of 50 grams of glucose + 15 grams of protein.
Interventions
DIETARY_SUPPLEMENTHigh-fat, high-energy diet
Participants consumed a diet that was high in fat and high in energy
Sponsors
Loughborough University
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
15 healthy individuals were assessed before and after consuming a high-fat, high-energy diet for 7 days.
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Aged between 18 and 40 years * Physically active * Non-smoker * Not taking any medication known to interfere with the study * No history of cardiovascular disease or diabetes * Weight stable for the last 6 months
Exclusion criteria
* Outside of age range * Sedentary * Smoker * Taking medication known to interfere with the study * History of cardiovascular disease or diabetes * Unstable weight within last 6 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in glucose concentration | Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart) | Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Glucose was reported as mmol/L. |
| Change in insulin concentration | Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart) | Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Insulin was reported as pmol/L. |
| Change in glucose kinetics (i.e., rate of appearance and disappearance) assessed using isotope tracers | Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart) | Glucose kinetics were assessed the day before and the day after the 7 day high-fat diet. Data expressed as umol/kg/min. |
| Change in skeletal muscle insulin signalling | Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart) | AKT and AS160 phosphorylation was determined by Western blotting of skeletal muscle. Data are expressed as arbitrary units. |
| Change in markers of microvascular function | Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart) | endothelial nitric oxide synthase was determined in terminal arterioles by quantitative immunofluorescence microscopy that was performed on sections of skeletal muscle tissue. Data presented as arbitrary units. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change intramuscular triglyceride content | Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart) | Intramuscular triglyceride content was determined by quantitative immunofluorescence microscopy that was performed on sections of skeletal muscle tissue. Data presented as arbitrary units. |
Outcome results
None listed