Healthy
Conditions
Brief summary
The impact of two days bed rest versus two days of habitual activity on insulin sensitivity and cumulative muscle protein synthesis will be investigated in healthy young males
Detailed description
To study the mechanisms underlying inactivity-induced muscle atrophy, the impact of two days bed rest versus two days of habitual activity on insulin sensitivity and cumulative muscle protein synthesis will be investigated in healthy young males
Interventions
BEHAVIORALHabitual activity
Two days of habitual physical activity, under normal free-living conditions
BEHAVIORALBed rest
Two days of bed rest, to induce whole-body physical inactivity
Sponsors
University of Exeter
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Participants will firstly undergo 2 days of habitual physical activity, followed by 2 days of bed rest
Eligibility
Sex/Gender
MALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Male * Aged 18-40
Exclusion criteria
* Smoker * Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes) * Any diagnosed cardiovascular disease (e.g. deep vein thrombosis or hypertension) * Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism). * A personal or family history of thrombosis, epilepsy, seizures or schizophrenia. * Any known disorders in muscle metabolism * Regular use of nutritional supplements (i.e. ingestion more than twice per week during the last month) * Metallic implants (including heart pacemaker, cochlear implants, medication pumps, surgical clips, plates or screws). * Allergy to lidocaine * Recent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in whole-body insulin sensitivity | During 2 days of habitual physical activity and 2 days of bed rest. Blood glucose and insulin are monitored for 2 hours following ingestion of a standardized meal | Change in whole-body insulin sensitivity via meal tolerance test |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in cumulative muscle protein synthesis | During 2 days of habitual physical activity and 2 days of bed rest | Cumulative muscle protein synthesis via deuterium oxide ingestion. The incorporation of deuterated alanine in muscle is expressed relative to body water enrichments, to obtain muscle protein synthesis in %/day |
| Change in continuous glucose | Continuous glucose, during the 2 days of habitual activity and 2 days of bed rest | Change in continuous glucose via continuous glucose monitoring. |
| Change in muscle volume | Immediately before and immediately after the 2-day bed rest period | Change in muscle volume via MRI |
Outcome results
None listed