Kidney Stone
Conditions
Keywords
dietary oxalate, diet therapy
Brief summary
The purpose of this study is to evaluate mitochondrial function in white blood cells and platelets from healthy individuals following dietary oxalate intake.
Detailed description
Inflammation and dietary oxalate have been shown to play an important role in the development of kidney stones. Oxalate is a small molecule found in plants and plant-derived food. Individuals that have high oxalate intake have an increased risk of developing kidney stones. The co-investigators have previously shown that healthy subjects that consume dietary oxalate have increased plasma and urine oxalate. White blood cells are essential for the immune response and rely on the mitochondria to carry out important cell functions. The Principal Investigator's current research shows that patients with calcium oxalate kidney stones have decreased mitochondrial function in their immune cells. Using the expertise of the investigators, this study will test whether oxalate has a direct effect on mitochondrial function in immune cells from healthy subjects. This information could help us understand the role of oxalate on the immune system during kidney stone pathogenesis.
Interventions
DIETARY_SUPPLEMENTBreakfast
a prepared meal consisting of pastry and 2 cooked egg whites
DIETARY_SUPPLEMENTSpinach smoothie
a prepared blended spinach smoothie, consisting of banana, avocado, orange juice, and spinach
DIETARY_SUPPLEMENTKale smoothie
prepared blended kale smoothie, consisting of banana, avocado, orange juice, and kale
DIETARY_SUPPLEMENTBlended smoothie
a prepared blended smoothie, consisting of banana, avocado, and orange juice
DIETARY_SUPPLEMENTV spinach smoothie
a prepared blended spinach smoothie, consisting of banana, avocado, orange juice, and a varying amount (low, medium, high) of spinach
DIETARY_SUPPLEMENTSodium oxalate drink
a prepared sodium oxalate drink
Sponsors
University of Alabama at Birmingham
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* Mentally competent adults who are able to read and comprehend the consent form, written in English * Male or Female * Age 18 to 70 years * Non-tobacco users * BMI between 19 and 27 * Willingness to abstain from vigorous exercise during study period * Normal blood comprehensive metabolic panel
Exclusion criteria
* Mentally incompetent adults who are unable to read or comprehend the consent form, written in English * Pregnant females * Active medical problems * History of kidney stones * Any medical disorder that could influence absorption or excretion of oxalate * BMI less than 19 or greater than 27 * Tobacco users * Current use of medications or dietary supplements
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in monocyte cellular energetics after 5 hours | 0 hours - 5 hours | Cellular bioenergetics (oxygen consumption rate) will be measured in isolated monocytes from human blood using a Seahorse XF Analyzer machine. Monocyte cellular bioenergetics will be measured before and after consuming a spinach smoothie (pre- and post-loads; Time 0 hours and 5 hours) to assess a change in monocyte cellular energetics after 5 hours. |
Countries
United States
Outcome results
None listed