Sickle Cell Disease
Conditions
Brief summary
The study consists in collecting umbilical cord blood cells from newborns at risk of sickle cell disease, to perform laboratory experiments aiming to characterize the cells with HbS/HbS mutation, to develop methods to prepare, to gene-modify and to preserve these cells.
Detailed description
Pregnant individuals carrying at least one HbS allele will be included in the study to collect the umbilical cord blood of the child at birth. Collected cells will be used anonymously for genetic and bioexperimental laboratory research, aiming to develop autologous gene therapy for sickle cell disease.
Interventions
None listed
Sponsors
Centre Hospitalier Sud Francilien
Institut National de la Santé Et de la Recherche Médicale, France
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
* Pregnant women, giving birth at CHSF and consenting to the collect and study of placental blood after delivery * Age 18 to 45 years * Biological testing of the participant includes hemoglobin electrophoresis and shows at least one HbS allele
Exclusion criteria
* Lack of written consent * Minors (not 18 years old) or under guardianship * Diseases : HIV, Hepatitis B, Hepatitis C or HTLV (Human T Leukemia Virus).
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Number of samples with HbS/HbS genotype | 3 years | Measured by DNA sequencing |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Number of samples with bio-experimental data | 4 years | Consisting of cellular characterization, transduction and cell processing data |
Countries
France
Outcome results
None listed