Diet Modification, Muscle Mass, Protein Breakdown, Protein Intake, Protein Synthesis, Sarcopenia
Conditions
Keywords
Dietary protein, Sarcopenia, Protein turnover, Muscle protein
Brief summary
The project examines over several days how the distribution pattern of dietary protein stimulate and affect the protein turnover of important proteins in the aging perspective, such as skeletal muscle proteins.
Interventions
OTHERDistribution pattern of dietary proteins (minced meat)
Study effect of an even or skewed distribution pattern of dietary protein
Sponsors
Bispebjerg Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
TRIPLE (Caregiver, Investigator, Outcomes Assessor)
Intervention model description
Randomized controlled
Eligibility
Sex/Gender
ALL
Age
65 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* Men and women * Between 65-80 years of age
Exclusion criteria
* BMI above 30 * Smokers * Vegetarians * Diabetes or other metabolic diseases * Medical treatment which affects protein turnover * Gastro-intestinal diseases, reduced kidney or liver function, or chronic inflammation diseases * Elevated blood-pressure or signs of atherosclerosis * More than 5 hours of regular exercise each week
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Muscle protein synthesis | 3 days | Myofibrillar FSR measured by deuterated water technique, by which deuterium labeled alanine incorporation into myofibrillar muscle protein synthesis can be measured. |
| Net protein balance | 10 hours | Whole body net protein balance measured during 10 hours acute study. Measured by the application of stable amino acid labeled phenylalanine and tyrosine tracers, by which the protein synthesis, phenylalanine oxidation and protein breakdown can be calculated. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Protein synthesis | 10 hours | Whole body protein synthesis (%/hour) measured during acute study, by the use of stabel amino acid tracer technique. |
| Protein breakdown | 10 hours | Whole body protein breakdown (%/hour) measured during acute study, by the use of stabel amino acid tracer technique. |
| Phenylalanine oxidation | 10 hours | Phenylalanine oxidation measured during acute study |
| D5-phenylalanine incorporation | 3 days | D5-phenylalanine incorporation into myofibrillar proteins. Dietary meat proteins are intrinsically labeled with D5-phenylalanine |
| Signaling pathways in skeletal muscle | 10 hours | Intracellular signaling pathways related to protein turnover. Measured by Western blotting technique from the muscle tissue biopsies taken at 2 hours and 12 hours into the acute study. |
| Plasma insulin | 12 hours | Plasma insulin concentration during acute study |
| D5-phenylalanine appearance | 12 hours | Plasma D5-phenylalanine enrichment after intake of D5-phenylalanine intrinsically labelled meat proteins |
| Muscle protein synthesis | 10 hours | Myofibrillar protein synthesis (%/hour) measured during 10 hours of the acute study. Calculated from the difference in D8-phenylalanine incorporation from the muscle biopsies taken at 2 hours and 12 hours into the stable amino acid tracer infusion. |
Countries
Denmark
Outcome results
None listed